Floyd W E, Zaleske D J, Schiller A L, Trahan C, Mankin H J
J Bone Joint Surg Am. 1987 Feb;69(2):185-90.
Although the formation of a secondary center of ossification is often compared with that of the primary center, there are striking differences between these processes. In the formation of the primary center, vascular invasion is always associated with the maturation of chondrocytes, whereas vascularization of the epiphysis can proceed in two different ways. In some species, the epiphysis is vascularized by cartilage canals before the appearance of the secondary center. However, in the mouse, the distal femoral epiphysis is vascularized by peripheral vascular invasion without pre-existing cartilage canals. Histological study of serial sections and studies of vascularization by injection with India ink demonstrated the relationship between hypertrophic chondrocyte formation, vascular invasion, and the formation of the secondary center of ossification in the murine distal femoral epiphysis.
尽管骨化次级中心的形成常与初级中心的形成相比较，但这些过程之间存在显著差异。在初级中心的形成过程中，血管侵入总是与软骨细胞的成熟相关，而骨骺的血管化可以通过两种不同方式进行。在某些物种中，在次级中心出现之前，骨骺由软骨管进行血管化。然而，在小鼠中，股骨远端骨骺通过外周血管侵入进行血管化，不存在预先存在的软骨管。对连续切片的组织学研究以及通过注射印度墨汁进行血管化研究，证实了小鼠股骨远端骨骺中肥大软骨细胞形成、血管侵入与骨化次级中心形成之间的关系。
