鉴定和描述大飞扬草乳浆中的蛋白质。
Identification and characterization of Euphorbia nivulia latex proteins.
机构信息
Department of Biochemistry, National Institute for Research in Reproductive Health, ICMR, Jehangir Merwanji Street, Parel, Mumbai 400 012, Maharashtra, India; Faculty of Science, Department of Biotechnology, Moolji Jaitha College, North Maharashtra University, Jalgaon 425 002, Maharashtra, India.
Faculty of Science, Department of Biotechnology, Moolji Jaitha College, North Maharashtra University, Jalgaon 425 002, Maharashtra, India.
出版信息
Int J Biol Macromol. 2014 Mar;64:193-201. doi: 10.1016/j.ijbiomac.2013.12.006. Epub 2013 Dec 12.
The protein profile of latex of Euphorbia nivulia Buch.-Ham. is established. Three new proteins viz., Nivulian-I, II and III have been purified to homogeneity from the latex. The relative molecular masses of Nivulian-I, II and III are 31,486.985, 43,670.846 and 52,803.470 Da respectively. Nivulian-I is a simple type of protein while Nivulian-II and III are glycoproteins. Peptide mass fingerprint analysis revealed peptides of these proteins match with Tubulin alpha-1 chain of Eleusine indica, Maturase K of Banksia quercifolia and hypothetical protein of Zea mays respectively. Tryptic digestion profile of Nivulian-I, II and III, infer the exclusive nature of latex origin proteins and may be new and are additive molecules in the dictionaries of phytoproteins or botany. This is the first of its kind, regarding characterization and validation of Nivulian-I, II and III with respect to peptide sequencing.
建立了Euphorbia nivulia Buch.-Ham.乳胶的蛋白质图谱。已从乳胶中纯化为三种新的蛋白质，即Nivulian-I、II 和 III。Nivulian-I、II 和 III 的相对分子质量分别为 31,486.985、43,670.846 和 52,803.470 Da。Nivulian-I 是一种简单的蛋白质，而 Nivulian-II 和 III 是糖蛋白。肽质量指纹图谱分析表明，这些蛋白质的肽与 Eleusine indica 的微管蛋白 alpha-1 链、Banksia quercifolia 的 maturase K 和 Zea mays 的假设蛋白相对应。Nivulian-I、II 和 III 的胰蛋白酶消化图谱推断出乳胶来源蛋白质的独特性质，可能是新的和附加的植物蛋白或植物学词典中的分子。这是首次对 Nivulian-I、II 和 III 进行肽序列测定的特征和验证。
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