Nivulian-II：一种来自飞扬草乳汁的新型凝乳半胱氨酸蛋白酶。
Nivulian-II a new milk clotting cysteine protease of Euphorbia nivulia latex.
作者信息
Badgujar Shamkant B, Mahajan Raghunath T
机构信息
Department of Biochemistry, National Institute for Research in Reproductive Health (ICMR), Jehangir Merwanji Street, Parel, Mumbai 400012, Maharashtra, India; Faculty of Science, Department of Biotechnology, Moolji Jaitha College, North Maharashtra University, Jalgaon 425002, Maharashtra, India.
Faculty of Science, Department of Biotechnology, Moolji Jaitha College, North Maharashtra University, Jalgaon 425002, Maharashtra, India.
出版信息
Int J Biol Macromol. 2014 Sep;70:391-8. doi: 10.1016/j.ijbiomac.2014.07.022. Epub 2014 Jul 17.
Nivulian-II, new milk clotting cysteine protease has been purified from the latex of Euphorbia nivulia Buch.-Ham. Nivulian-II is a monomeric protein with an apparent molecular mass 43670.846 Da. It presents its optimum activity at pH 6.3 and temperature of 50°C. The enzyme was strongly inhibited by common thiol-blocking reagents thereby indicating that it belongs to cysteine protease family. Nivulian-II is a type of glycoprotein and its pI is 3.4. The N-terminal amino acid sequence of Nivulian-II is DFPPNTCCCICC. This sequence showed relatively low homology with several other proteases of Euphorbian plants, suggesting that the isolated enzyme is a new cysteine protease.
从大戟科植物泽漆（Euphorbia nivulia Buch.-Ham.）的乳汁中纯化出了一种新型凝乳半胱氨酸蛋白酶——Nivulian-II。Nivulian-II是一种单体蛋白，表观分子量为43670.846道尔顿。它在pH 6.3和50°C温度下呈现最佳活性。该酶受到常见硫醇阻断剂的强烈抑制，因此表明它属于半胱氨酸蛋白酶家族。Nivulian-II是一种糖蛋白，其等电点为3.4。Nivulian-II的N端氨基酸序列为DFPPNTCCCICC。该序列与大戟科植物的其他几种蛋白酶的同源性相对较低，表明分离出的这种酶是一种新型半胱氨酸蛋白酶。
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