Faculty of Bioenvironmental Science, Kyoto Gakuen University, 1-1 Nanjo, Sogabe, Kameoka 621-8555, Japan.
Faculty of Bioenvironmental Science, Kyoto Gakuen University, 1-1 Nanjo, Sogabe, Kameoka 621-8555, Japan.
Insect Biochem Mol Biol. 2014 Feb;45:51-7. doi: 10.1016/j.ibmb.2013.11.006. Epub 2013 Dec 11.
De novo biosynthesis of linoleic acid (LA) and its conversion to (Z,Z)-6,9-heptadecadiene were examined in Carpoglyphus lactis (Acarina, Carpoglyphidae). Experiments involving (13)C-administration using [1-(13)C]-d-glucose revealed that (13)C atoms were incorporated into LA of total lipid extracted from the mite, resulting in labeling of all even-numbered carbons. This result demonstrated that LA was produced from (13)C-labeled acetyl-CoA, which is indicative of direct de novo biosynthesis. In these feeding experiments involving [1-(13)C]-D-glucose, (13)C atoms were also incorporated into (Z,Z)-6,9-heptadecadiene, which is one of the major secretory components in the mite. The labeling pattern of (Z,Z)-6,9-heptadecadiene at odd-numbered carbons agreed well with that of LA after loss of the carboxyl carbon. It was concluded that the mites could stably convert LA into (Z,Z)-6,9-heptadecadiene without the dietary requirement of this essential fatty acid.
在乳木虱（节肢动物门，乳木虱科）中研究了亚油酸（LA）的从头生物合成及其转化为（Z，Z）-6,9-十七碳二烯。使用[1-（13）] -d-葡萄糖进行（13）C 给药的实验表明，（13）C 原子掺入了从螨中提取的总脂质中的 LA 中，导致所有偶数位碳被标记。这一结果表明 LA 是由（13）C 标记的乙酰辅酶 A 产生的，这表明其是直接从头合成的。在这些涉及[1-（13）] -D-葡萄糖的喂养实验中，（13）C 原子也被掺入螨类中主要的分泌成分之一（Z，Z）-6,9-十七碳二烯中。（Z，Z）-6,9-十七碳二烯奇数位碳的标记模式与失去羧基碳后的 LA 非常吻合。因此可以得出结论，螨虫可以在不需要这种必需脂肪酸的情况下稳定地将 LA 转化为（Z，Z）-6,9-十七碳二烯。
