Pfeffer L M, Tamm I
J Interferon Res. 1986 Oct;6(5):551-6. doi: 10.1089/jir.1986.6.551.
Addition of the lectin concanavalin A (ConA) to HeLa-S3 tumor cells in culture stimulates calcium exchange and uptake. Pretreatment of the cells with interferon-beta (IFN-beta) at a concentration of 640 U/ml for 24 h markedly inhibits this ConA-stimulated calcium exchange and uptake, but not the basal level of calcium exchange. These findings suggest that a number of IFN effects on cell function may reflect an impaired ability of IFN-treated cells to respond to molecules such as growth factors and peptide hormones with increased calcium fluxes.
在培养的HeLa-S3肿瘤细胞中添加凝集素伴刀豆球蛋白A（ConA）可刺激钙交换和摄取。用浓度为640 U/ml的干扰素-β（IFN-β）预处理细胞24小时，可显著抑制ConA刺激的钙交换和摄取，但不影响钙交换的基础水平。这些发现表明，IFN对细胞功能的许多影响可能反映出经IFN处理的细胞对诸如生长因子和肽激素等分子作出钙通量增加反应的能力受损。
