Dinc Emine, Ramundo Silvia, Croce Roberta, Rochaix Jean-David
Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, 1081 HV Amsterdam, The Netherlands.
Departments of Molecular Biology and Plant Biology, University of Geneva, 30, Quai Ernest Ansermet, 1211 Geneva, Switzerland.
Biochim Biophys Acta. 2014 Sep;1837(9):1548-52. doi: 10.1016/j.bbabio.2013.11.020. Epub 2013 Dec 12.
A repressible/inducible chloroplast gene expression system has been used to conditionally inhibit chloroplast protein synthesis in the unicellular alga Chlamydomonas reinhardtii. This system allows one to follow the fate of photosystem II and photosystem I and their antennae upon cessation of chloroplast translation. The main results are that the levels of the PSI core proteins decrease at a slower rate than those of PSII. Amongst the light-harvesting complexes, the decrease of CP26 proceeds at the same rate as for the PSII core proteins whereas it is significantly slower for CP29, and for the antenna complexes of PSI this rate is comprised between that of CP26 and CP29. In marked contrast, the components of trimeric LHCII, the major PSII antenna, persist for several days upon inhibition of chloroplast translation. This system offers new possibilities for investigating the biosynthesis and turnover of individual photosynthetic complexes in the thylakoid membranes. This article is part of a special issue entitled: photosynthesis research for sustainability: keys to produce clean energy.
一种可阻遏/可诱导的叶绿体基因表达系统已被用于有条件地抑制单细胞藻类莱茵衣藻中的叶绿体蛋白质合成。该系统使人们能够追踪叶绿体翻译停止后光系统II和光系统I及其天线的命运。主要结果是，PSI核心蛋白水平下降的速度比PSII慢。在捕光复合物中，CP26的下降速度与PSII核心蛋白相同，而CP29的下降速度明显较慢，对于PSI的天线复合物，其下降速度介于CP26和CP29之间。明显不同的是，三聚体LHCII（主要的PSII天线）的成分在叶绿体翻译受到抑制后会持续存在数天。该系统为研究类囊体膜中单个光合复合物的生物合成和周转提供了新的可能性。本文是名为“可持续性光合作用研究：生产清洁能源的关键”的特刊的一部分。
