Morens D M, Venkateshan C N, Halstead S B
J Gen Virol. 1987 Jan;68 ( Pt 1):91-8. doi: 10.1099/0022-1317-68-1-91.
Nineteen monoclonal antibodies produced to dengue type 4 virus (DEN-4) strain 4328-S were tested for their ability to mediate antibody-dependent infection enhancement (ADE) with seven DEN-2 strains in P-388D1 mouse macrophage-like cells. In this first study of the distribution of enhancing epitopes on multiple DEN-2 strains reacted with monoclonal antibodies to a different serotype (DEN-4), DEN-4 monospecific antibodies produced ADE with DEN-2 viruses, indicating the presence of DEN-4-like determinants on DEN-2 viruses. Analysis differentiated at least one and possibly more DEN-2 strain subgroups, one of which (isolates AHF-110 and AHF-191) was previously identified by DEN-2 monoclonal antibody analysis. The study demonstrates the heterogeneous distribution of dengue complex and DEN-4 epitopes on DEN-2 strains. Monoclonal antibodies are valuable tools for study of the biology of ADE and its relation to dengue shock syndrome.
针对登革4型病毒（DEN-4）4328-S株产生的19种单克隆抗体，在P-388D1小鼠巨噬细胞样细胞中，检测了它们介导7种DEN-2株抗体依赖性感染增强（ADE）的能力。在第一项针对与不同血清型（DEN-4）单克隆抗体反应的多种DEN-2株上增强表位分布的研究中，DEN-4单特异性抗体与DEN-2病毒产生了ADE，表明DEN-2病毒上存在DEN-4样决定簇。分析区分出至少一个且可能更多的DEN-2株亚组，其中一个亚组（分离株AHF-110和AHF-191）先前已通过DEN-2单克隆抗体分析鉴定。该研究证明了登革病毒复合体和DEN-4表位在DEN-2株上的异质性分布。单克隆抗体是研究ADE生物学及其与登革休克综合征关系的有价值工具。
