Bloomquist E I, Kream R M
J Pharmacol Exp Ther. 1987 Feb;240(2):523-8.
The role of neurotransmitter release in generation of the contractile response to leukotriene D4 (LTD4) by guinea pig ileum longitudinal smooth muscle preparations was studied. The contractile response to LTD4 was inhibited partially by tetrodotoxin (TTx) with early portions of the contraction being more sensitive to inhibition by TTx. This finding suggests that part of the LTD4 contraction is mediated by release of a neurotransmitter as a result of activation of Na+-dependent action potentials. Three approaches, study of the effects of substance P (SP) desensitization and of the effects of a SP antagonist on the LTD4 response and direct measurement of SP release were used to evaluate the possibility that SP is released by LTD4. Partial inhibition of the LTD4 contraction by SP desensitization, and by the SP antagonist, as well as direct measurement of TTx sensitive SP release in the presence of LTD4, indicates that SP is released from neurons by LTD4 in guinea pig ileum.
研究了神经递质释放在豚鼠回肠纵行平滑肌制剂对白三烯D4（LTD4）产生收缩反应中的作用。河豚毒素（TTx）可部分抑制对LTD4的收缩反应，收缩早期部分对TTx的抑制更敏感。这一发现表明，LTD4收缩的一部分是由依赖Na+的动作电位激活导致神经递质释放所介导的。采用三种方法来评估LTD4是否释放P物质（SP），即研究SP脱敏的作用、SP拮抗剂对LTD4反应的作用以及直接测量SP释放。SP脱敏和SP拮抗剂对LTD4收缩的部分抑制，以及在存在LTD4的情况下直接测量对TTx敏感的SP释放，表明在豚鼠回肠中，LTD4可使神经元释放SP。
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