糖尿病动物模型中自体脂肪移植存活率降低：一项初步研究。
Impaired survival of autologous fat grafts by diabetes mellitus in an animal model: a pilot study.
机构信息
Departments of Plastic and Reconstructive Surgery and Internal Medicine, Soonchunhyang University of Medicine, Bucheon, Korea.
出版信息
Aesthet Surg J. 2014 Jan 1;34(1):168-74. doi: 10.1177/1090820X13515675. Epub 2013 Dec 13.
BACKGROUND
Many surgeons are reluctant to perform fat grafting in patients with diabetes because soft-tissue healing is impaired, but no previous study has reported data-driven outcomes of fat grafting in subjects with diabetes.
OBJECTIVE
The authors investigate whether diabetes affected survival and complication rates of autologous fat grafting (AFG) in Sprague-Dawley (SD) and Otsuka Long-Evans Tokushima Fatty (OLETF) rats.
METHODS
In total, 16 male SD and 16 male OLETF rats were prepared for AFG. Fat tissue was harvested from the inguinal fat pad and cut into 1-g (800-1250 mg) sections. Each rat received fat in a subcutaneous pocket in the paraspinal area. The grafted fat tissue was measured by ultrasonography at 30, 60, and 90 days after implantation and evaluated by histological analyses.
RESULTS
Ultrasonographic analysis indicated that compared with the SD group, the AFG survival rate was lower in OLETF rats (40.52% vs 53.38%), and the failure rate was higher in OLETF rats (31.25% vs 6.25%). The histological analysis indicated that compared with the OLETF group, adipocytes were more dense (3.18 vs 2.33; P = .025), cyst formation occurred less frequently (3.00 vs 3.61; P = .030), and more capillaries were formed (2.31/field vs 1.61/field; P = .001) in the SD rat group.
CONCLUSIONS
Diabetes causes marked deterioration in the survival and quality of AFG in the diabetic rat model.
背景
许多外科医生不愿意在糖尿病患者中进行脂肪移植，因为软组织愈合受损，但以前没有研究报告过糖尿病患者自体脂肪移植（AFG）的数据驱动结果。
目的
作者研究糖尿病是否影响 Sprague-Dawley（SD）和 Otsuka Long-Evans Tokushima Fatty（OLETF）大鼠自体脂肪移植（AFG）的存活率和并发症发生率。
方法
总共准备了 16 只雄性 SD 和 16 只雄性 OLETF 大鼠进行 AFG。从腹股沟脂肪垫中采集脂肪组织，切成 1g（800-1250mg）的切片。每只大鼠在脊柱旁区域的皮下口袋中接受脂肪移植。在植入后 30、60 和 90 天通过超声检查测量移植的脂肪组织，并进行组织学分析。
结果
超声分析表明，与 SD 组相比，OLETF 大鼠的 AFG 存活率较低（40.52%对 53.38%），失败率较高（31.25%对 6.25%）。组织学分析表明，与 OLETF 组相比，SD 大鼠组的脂肪细胞更密集（3.18 对 2.33；P =.025），囊泡形成较少（3.00 对 3.61；P =.030），形成的毛细血管更多（2.31/视野对 1.61/视野；P =.001）。
结论
糖尿病导致糖尿病大鼠模型中 AFG 的存活率和质量明显恶化。
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