Manikonda Pavan K, Rajendra Pilankatta, Devendranath D, Gunasekaran B, Aradhya Shivakumara R S, Sashidhar Rao B, Subramanyam Chivukula
Department of Biochemistry, University College of Science, Osmania University, Hyderabad-500 007, AP, India.
Gen Physiol Biophys. 2014;33(1):81-90. doi: 10.4149/gpb_2013059. Epub 2013 Dec 13.
The present investigation was conducted to understand the influence of long-term exposure of rats to extremely low frequency magnetic fields (ELF-MF), focusing on oxidative stress (OS) on different regions of rat's brain. Male Wistar rats (21-day-old) were exposed to ELF-MF (50 Hz; 50 and 100 µT) for 90 days continuously; hippocampal, cerebellar and cortical regions from rats were analyzed for (i) reactive oxygen species (ROS), (ii) metabolites indicative of OS and (iii) antioxidant enzymes. In comparison to control group rats, the rats that were continuously exposed to ELF-MF caused OS and altered glutathione (GSH/GSSG) levels in dose-dependent manner in all the regions of the brain. Accumulation of ROS, lipid peroxidation end products and activity of superoxide dismutase in different regions was in the descending order of cerebellum < hippocampus < cortex. Decrement in GSH/GSSG levels and increment in glutathione peroxidase activity were in the descending order of hippocampus < cerebellum < cortex. The continuous exposure to ELF-MF caused OS in all the examined regions of brain more significantly at 100 µT than at 50 µT. Varied influences observed in different regions of the brain, as documented in this study, may contribute to altered metabolic patterns in its related regions of the central nervous system, leading to aberrant neuronal functions.
本研究旨在了解大鼠长期暴露于极低频磁场（ELF-MF）的影响，重点关注大鼠大脑不同区域的氧化应激（OS）。将雄性Wistar大鼠（21日龄）连续90天暴露于ELF-MF（50Hz；50和100μT）；对大鼠的海马、小脑和皮质区域进行分析，检测（i）活性氧（ROS）、（ii）指示OS的代谢物和（iii）抗氧化酶。与对照组大鼠相比，连续暴露于ELF-MF的大鼠在大脑所有区域均引起OS，并以剂量依赖方式改变谷胱甘肽（GSH/GSSG）水平。不同区域ROS的积累、脂质过氧化终产物和超氧化物歧化酶的活性顺序为小脑<海马<皮质。GSH/GSSG水平的降低和谷胱甘肽过氧化物酶活性的增加顺序为海马<小脑<皮质。连续暴露于ELF-MF在100μT时比在50μT时更显著地在大脑所有检查区域引起OS。如本研究所述，在大脑不同区域观察到的不同影响可能导致中枢神经系统相关区域代谢模式的改变，从而导致神经元功能异常。
