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高压氧通过下调 HMGB1/NF-κB 的表达缓解实验性（脊髓）损伤。

Hyperbaric oxygen alleviates experimental (spinal cord) injury by downregulating HMGB1/NF-κB expression.

机构信息

*Department of Hyperbaric Oxygen, Beijing Chao-yang Hospital, Capital Medical University, Beijing, China †Department of Orthopaedics, Shen Zhou Hospital Affiliated to Shenyang Medical College, Shenyang, China ‡Department of Hyperbaric Oxygen, Civil Aviation General Hospital, Beijing, China; and §Department of Orthopaedics, Beijing Chao-yang Hospital, Capital Medical University, Beijing, China.

出版信息

Spine (Phila Pa 1976). 2013 Dec 15;38(26):E1641-8. doi: 10.1097/BRS.0000000000000005.

DOI:10.1097/BRS.0000000000000005

PMID:24335635

Abstract

STUDY DESIGN

We presented an insight into the effect of hyperbaric oxygen (HBO) on spinal cord injury (SCI), aiming to uncover the dynamics of high-mobility group protein B1 (HMGB1) and nuclear factor κB (NF-κB) after HBO intervention in rats with acute SCI.

OBJECTIVE

Prognosis of SCI is directly linked with the control of secondary injury, in which the inflammatory response plays a leading role. HBO therapy can reduce this secondary damage to the spinal cord. We used an animal model to characterize the therapeutic effect of HBO on SCI.

SUMMARY OF BACKGROUND DATA

A growing number of studies have confirmed that HBO has gradually become an indispensable element after SCI in reducing neurological disorders, and improving the physical function and quality of life of patients. The role of HBO in the process of HMGB1/NF-κB-related secondary inflammatory responses in SCI has yet to be characterized.

METHODS

Rats were randomly categorized into sham, sham + HBO, SCI, and SCI + HBO groups. The expression levels of HMGB1 and NF-κB were measured at days 1, 3, 7, and 14 after SCI.

RESULTS

After SCI, significant increases in mRNA and protein expression were observed for both HMGB1 and NF-κB (P< 0.01) compared with sham group. HMGB1 mRNA and protein expression levels were decreased after HBO intervention. The decreases were significant at days 7 and 14 (P< 0.05) post-HBO. In the SCI + HBO group, the significant decreases in NF-κB mRNA and protein expression levels were also observed at days 3, 7, and 14 (P< 0.05). After HBO intervention, a significant increase was seen in the Basso, Beattie, and Bresnahan score at days 7 and 14 (P< 0.05).

CONCLUSION

HBO intervention may reduce the secondary damage of SCI caused by inflammatory responses via downregulating the expression of HMGB1/NF-κB, and promoting the repair of neurological function.

LEVEL OF EVIDENCE

N/A.

摘要

研究设计

我们介绍了高压氧（HBO）对脊髓损伤（SCI）的影响，旨在揭示急性 SCI 大鼠 HBO 干预后高迁移率族蛋白 B1（HMGB1）和核因子κB（NF-κB）的动态变化。

目的

SCI 的预后与继发性损伤的控制直接相关，其中炎症反应起主导作用。HBO 治疗可以减少脊髓的这种继发性损伤。我们使用动物模型来描述 HBO 对 SCI 的治疗作用。

背景资料概要

越来越多的研究证实，HBO 在减少神经功能障碍方面已逐渐成为 SCI 后不可或缺的因素，并改善了患者的身体功能和生活质量。HBO 在 SCI 中与 HMGB1/NF-κB 相关的继发性炎症反应过程中的作用尚未得到描述。

方法

大鼠随机分为假手术组、假手术+HBO 组、SCI 组和 SCI+HBO 组。在 SCI 后第 1、3、7 和 14 天测量 HMGB1 和 NF-κB 的表达水平。

结果

与假手术组相比，SCI 后 HMGB1 和 NF-κB 的 mRNA 和蛋白表达均显著增加（P<0.01）。HBO 干预后 HMGB1 mRNA 和蛋白表达水平降低。HBO 后第 7 和 14 天下降有统计学意义（P<0.05）。在 SCI+HBO 组中，在第 3、7 和 14 天也观察到 NF-κB mRNA 和蛋白表达水平的显著降低（P<0.05）。HBO 干预后，第 7 和 14 天 Basso、Beattie 和 Bresnahan 评分均显著升高（P<0.05）。