Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, China.
Eur Rev Med Pharmacol Sci. 2013 Dec;17(23):3169-77.
To investigate the effects of systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndromes (MODS) on human peripheral blood endothelial progenitor cells.
Twenty patients admitted to Changhai Hospital, Second Military Medical University, from February, 2011 to November, 2011 were recruited consecutively. The serum samples were collected from the twenty patients who were divided into four groups as following: normal group, post-traumatic group without SIRS, post-traumatic group with SIRS, and post-traumatic group with MODS. Endothelial progenitor cells (EPCs) were isolated from peripheral blood of healthy subjects by using density gradient centrifugation and the effect of the serum on EPCs was detected after stimulating by the serum samples for 0, 6, 12, 24, and 36 h.
Compared with the normal group, the proliferation of EPCs was significantly increased in a time-independent manner in the other three groups, especially in the SIRS serum treated group. The expression of pro-inflammation cytokines was increased in the other three groups compared with the normal group, but the expression of IL-10 in the normal group was higher than the other groups.
Oxidative stress balance was also broken as the disease progressed. Serum from patients with sepsis could influence proliferation and the inflammation and oxidative stress states of EPCs.
探讨全身炎症反应综合征（SIRS）和多器官功能障碍综合征（MODS）对人外周血内皮祖细胞的影响。
连续入选 2011 年 2 月至 2011 年 11 月第二军医大学长海医院收治的 20 例患者。采集 20 例患者的血清标本，根据是否发生 SIRS 和 MODS 将患者分为四组：正常组、未发生 SIRS 的创伤后组、发生 SIRS 的创伤后组和发生 MODS 的创伤后组。采用密度梯度离心法从健康受试者外周血中分离内皮祖细胞（EPCs），用血清刺激 EPCs 0、6、12、24 和 36 h 后，检测血清对 EPCs 的影响。
与正常组相比，其他三组 EPC 增殖均呈时间依赖性显著增加，其中 SIRS 血清处理组最为明显。与正常组相比，其他三组促炎细胞因子的表达均增加，但正常组的 IL-10 表达高于其他各组。
随着疾病的进展，氧化应激平衡也被打破。脓毒症患者的血清可影响 EPC 的增殖及炎症和氧化应激状态。
