激肽释放酶在人腮腺和肾脏中的定位：免疫组织化学与酶组织化学的比较研究

Localization of kallikreins in the human parotid gland and in the human kidney: a comparative study of immunohistochemistry and enzyme histochemistry.

作者信息

Kimura K, Moriya H

出版信息

Adv Exp Med Biol. 1986;198 Pt A:27-34. doi: 10.1007/978-1-4684-5143-6_4.

Abstract

Kallikreins were localized in the human parotid gland and in the human kidney from two different aspects of the enzyme: the enzyme antigenicity and the enzyme activity. In the human parotid gland, the kallikrein antigenicity and the enzyme activity were identical in their locations in the ductal cells. In the human kidney, however, the enzyme activity was revealed in the proximal tubule without any corresponding kallikrein antigenicity. Therefore, this activity may be due to esterases other than kallikreins. The Kallikrein antigenicity was found to be located in the basement membrane of tubules, in the interstitium as well as in some granules of the proximal tubule. These results are compatible with tissue kallikreins being formed in the ducts of the human parotid gland and suggest that circulating tissue kallikreins in the plasma are deposited in the human kidney.

摘要

激肽释放酶从酶的两个不同方面，即酶抗原性和酶活性，在人类腮腺和肾脏中定位。在人类腮腺中，激肽释放酶抗原性和酶活性在导管细胞中的位置相同。然而，在人类肾脏中，近端小管显示出酶活性，但没有任何相应的激肽释放酶抗原性。因此，这种活性可能归因于激肽释放酶以外的酯酶。发现激肽释放酶抗原性位于小管的基底膜、间质以及近端小管的一些颗粒中。这些结果与人类腮腺导管中形成组织激肽释放酶一致，并表明血浆中循环的组织激肽释放酶沉积在人类肾脏中。