Schütte B
Andrologia. 1986 Nov-Dec;18(6):567-9. doi: 10.1111/j.1439-0272.1986.tb01829.x.
Spermatozoa stained with toluidine blue-pyronine can be differentiated very rapid within one hour after liquefaction of the ejaculate. In contrast to the method of Testsimplets the slides can be stored for record purpose. Furthermore its quality is comparable with the common staining techniques of spermatozoa. Therefore it can be recommended in the routine practice.
用甲苯胺蓝-派洛宁染色的精子在精液液化后一小时内可很快鉴别出来。与Testsimplets方法不同的是,载玻片可用于存档。此外,其质量与精子常见染色技术相当。因此,可在常规操作中推荐使用。