Department of Abdominal Surgery, Fujian Provincial Cancer Hospital, Fuzhou, Fujian 350014, China; Provincial Clinical College, Fujian Medical University, Fuzhou, Fujian 350014, China.
Department of Oncology, Guizhou Cancer Hospital, Guiyang, Guizhou 550004, China; Provincial Clinical College, Fujian Medical University, Fuzhou, Fujian 350014, China.
Chin Med J (Engl). 2013;126(24):4747-51.
The ability of pneumoperitoneum in laparoscopic surgery to promote proliferation and metastasis of colorectal cancer has become a focus of research in the field of minimally invasive surgery. The aim of this research was to investigate the effect of CO2 pneumoperitoneum under different pressures and exposed times on the expression of chemokine receptors in colorectal carcinoma cells.
We constructed an in vitro pneumoperitoneum model. SW480 colon carcinoma cells were exposed to CO2 pneumoperitoneum under different pressures (6, 9, 12, and 15 mmHg) for 1, 2, and 4 hours. These cells were then cultivated under the same conditions as normal SW480 colon carcinoma cells without CO2 pneumoperitoneum (control group), treated at 37°C, and 5% CO2. The expression of the chemokine receptors CXC receptor 4 (CXCR4) and chemokine C receptor 7 (CCR7) was detected by immunocytochemistry and reverse transcriptase polymerase chain reaction after being cultivated for 0, 24, 48, and 72 hours.
Immunocytochemistry showed that CXCR4 expression in SW480 cells was significantly decreased in the 6, 9, 12, and 15 mmHg CO2 pneumoperitoneum-treated groups for the same exposure times compared with controls (P < 0.05). CCR7 expression in SW480 cells was significantly decreased in the 12 and 15 mmHg CO2 pneumoperitoneum-treated groups compared with controls (P < 0.05). CXCR4 and CCR7 expression increased up to the level of the control group after 24 and 48 hours (P > 0.05). If the CO2 pneumoperitoneum pressure increased, CXCR4 and CCR7 expression decreased at all exposure times. If the CO2 pneumoperitoneum exposure time prolonged, there were no significant differences in CXCR4 and CCR7 expression under the same pressure. Under all exposure times, CXCR4 and CCR7 mRNA expression was significantly decreased in the 6, 9, 12, and 15 mmHg CO2 pneumoperitoneum-treated groups (P < 0.05) compared with controls, and it increased up to the level of controls after being cultivated for 48 hours (P > 0.05). If the CO2 pneumoperitoneum pressure increased (with all exposure times) and exposure time prolonged (under the same pressure), there were no significant differences in CXCR4 and CCR7 expression.
CXCR4 and CCR7 expression is temporarily affected after continuous CO2 pneumoperitoneum treatment. The high pressure of CO2 pneumoperitoneum plays an important role in suppressing the expression of these chemokine receptors. Different lengths of time of exposure to a CO2 pneumoperitoneum-like environment do not change CXCR4 and CCR7 expression.
腹腔镜手术中的气腹能够促进结直肠癌细胞的增殖和转移，这已成为微创外科领域的研究热点。本研究旨在探讨不同压力和暴露时间的 CO2 气腹对结直肠癌细胞趋化因子受体表达的影响。
构建体外气腹模型。SW480 结肠癌细胞在不同压力（6、9、12 和 15mmHg）下分别暴露 1、2 和 4 小时，然后在相同条件下培养，与未进行 CO2 气腹的正常 SW480 结肠癌细胞（对照组）一起在 37℃、5%CO2 下培养。培养 0、24、48 和 72 小时后，通过免疫细胞化学和逆转录聚合酶链反应检测趋化因子受体 CXC 受体 4（CXCR4）和趋化因子 C 受体 7（CCR7）的表达。
免疫细胞化学显示，与对照组相比，相同暴露时间下，SW480 细胞在 6、9、12 和 15mmHg CO2 气腹处理组中 CXCR4 的表达明显降低（P<0.05）。与对照组相比，SW480 细胞中 CCR7 的表达在 12 和 15mmHg CO2 气腹处理组中明显降低（P<0.05）。24 和 48 小时后，CXCR4 和 CCR7 的表达增加至对照组水平（P>0.05）。如果 CO2 气腹压力增加，则在所有暴露时间下 CXCR4 和 CCR7 的表达均降低。如果 CO2 气腹暴露时间延长，在相同压力下 CXCR4 和 CCR7 的表达无显著差异。在所有暴露时间下，SW480 细胞中 6、9、12 和 15mmHg CO2 气腹处理组的 CXCR4 和 CCR7 mRNA 表达均明显低于对照组（P<0.05），培养 48 小时后表达增加至对照组水平（P>0.05）。如果 CO2 气腹压力升高（所有暴露时间）或暴露时间延长（相同压力），则 CXCR4 和 CCR7 的表达无显著差异。
连续 CO2 气腹处理后 CXCR4 和 CCR7 的表达暂时受到影响。CO2 气腹的高压对抑制这些趋化因子受体的表达起着重要作用。暴露于 CO2 气腹样环境的不同时间长度不会改变 CXCR4 和 CCR7 的表达。
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