Department of Comparative Anatomy, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 3, 10-727 Olsztyn, Poland.
Neuroscience. 2014 Feb 28;260:98-105. doi: 10.1016/j.neuroscience.2013.12.015. Epub 2013 Dec 14.
The study describes for the first time the colocalization pattern of calbindin (CB) and cocaine- and amphetamine-regulated transcript (CART) in the mammillary body (MB) and anterior thalamic nuclei (ATN) - structures connected in a topographically organized manner by the mammillothalamic tract (mtt). Immunohistochemical study was performed on fetal (E40, E50, E60), newborn (P0) and postnatal (P20, P80) brains of the guinea pig, but the coexistence pattern of the substances was invariable throughout the examined developmental stages. CB and CART colocalized in the perikarya of the lateral part of the medial mammillary nucleus (MMl), whereas in its medial part (MMm) only CB was detected. In the mtt, which originates from the MB, both the substances were present and colocalized in single fibers. Next, fibers from the mtt spread toward the ATN in a particular way: fibers containing CB ran to both the anteromedial thalamic nucleus (AM) and anteroventral thalamic nucleus (AV), while fibers containing CART ran mostly to the latter one. In the ventral part of AV, CB and CART colocalized vastly in the neuropil. The lateral mammillary nucleus and anterodorsal thalamic nucleus were virtually devoid of CB- and CART-positive structures. Based on the known connections between the MB and ATN, we conclude that the studied substances may cooperate in the MMl-AV part of the axis and CB plays a significant role in the MMm-AM part.
该研究首次描述了在乳头体(MB)和前丘脑核(ATN)中钙结合蛋白(CB)和可卡因-安非他命调节转录物(CART)的共定位模式 - 这些结构通过乳头丘脑束(mtt)以拓扑方式连接。对豚鼠胎儿(E40、E50、E60)、新生(P0)和新生(P20、P80)脑进行了免疫组织化学研究,但在整个检查的发育阶段,物质的共存模式都是不变的。CB 和 CART 在内侧乳头核(MMl)外侧部分的神经元中共定位,而在其内侧部分(MMm)仅检测到 CB。在起源于 MB 的 mtt 中,两种物质都存在并在单个纤维中共定位。接下来,来自 mtt 的纤维以特定的方式向 ATN 扩散:含有 CB 的纤维通向前内侧丘脑核(AM)和前腹侧丘脑核(AV),而含有 CART 的纤维主要通向后者。在 AV 的腹侧部分,CB 和 CART 在神经胶质中广泛共定位。外侧乳头核和前背侧丘脑核几乎没有 CB 和 CART 阳性结构。基于 MB 和 ATN 之间的已知连接,我们得出结论,研究的物质可能在 MMl-AV 轴的部分中合作,而 CB 在 MMm-AM 部分中发挥重要作用。