Ding Qian, Zhang Feng-qiu, Ma Yu-shi
Department of Periodontics, the Affiliated Beijing Stomatological Hospital of Capital Medical University, Beijing 100050, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2013 Dec 18;45(6):975-8.
To observe the effect of icariin on human periodontal ligament cells (hPDLCs) differentiation to osteoblast gene expression.
The fifth generation of the cultured hPDLCs was added with the concentration of 0.01 mg/L icariin, and the added osteogenic medium used as blank control group, alizarin red staining of icariin on human periodontal ligament cells was observed for 21 days; the 2, 4, and 6 days of Q-PCR quantitative analysis of icariin on human periodontal ligament cells were made for osteogenesis gene alkaline phosphatase (ALP), type I collagen and osteocalcin (OC) gene expression.
For the 21 days, alizarin red staining icariin group formed more mineralized nodules; on the 2nd, 4th, and 6th days, the group of icariin promoted the expression of ALP and OC mRNA, reached the peak value on day 6, compared with the control group with significant difference (20.15±6.67 vs. 7.90±0.71, 4.13±0.56 vs. 3.55±0.08, P<0.01). The second day, the highest expression of type I collagen appeared, then decreased gradually after, statistically compared with the control group (P<0.05).
Icariin can promote the human periodontal ligament cells differentiation to osteoblast, and promote the osteogenesis gene expression.
观察淫羊藿苷对人牙周膜细胞（hPDLCs）向成骨细胞分化过程中基因表达的影响。
将培养至第五代的hPDLCs加入浓度为0.01mg/L的淫羊藿苷，并加入成骨培养基作为空白对照组，观察淫羊藿苷对人牙周膜细胞进行茜素红染色21天的情况；对淫羊藿苷作用于人牙周膜细胞2、4、6天进行Q-PCR定量分析成骨相关基因碱性磷酸酶（ALP）、Ⅰ型胶原和骨钙素（OC）的基因表达情况。
21天时，淫羊藿苷组茜素红染色形成更多矿化结节；在第2、4、6天，淫羊藿苷组促进了ALP和OC mRNA的表达，在第6天达到峰值，与对照组相比有显著差异（20.15±6.67 vs. 7.90±0.71，4.13±0.56 vs. 3.55±0.08，P<0.01）。第2天时，Ⅰ型胶原表达最高，之后逐渐下降，与对照组相比有统计学差异（P<0.05）。
淫羊藿苷能促进人牙周膜细胞向成骨细胞分化，并促进成骨相关基因表达。
