Gupta Aradhana, Stockham Steven L
Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506.
J Am Vet Med Assoc. 2014 Jan 1;244(1):63-7. doi: 10.2460/javma.244.1.63.
To determine whether high serum bilirubin concentrations interfere with the measurement of serum total protein concentration by refractometry and to assess potential biases among refractometer measurements.
Evaluation study.
Sera from 2 healthy Greyhounds.
Bilirubin was dissolved in 0.1M NaOH, and the resulting solution was mixed with sera from 2 dogs from which food had been withheld to achieve various bilirubin concentrations up to 40 mg/dL. Refractometric total protein concentrations were estimated with 3 clinical refractometers. A biochemical analyzer was used to measure biuret assay-based total protein and bilirubin concentrations with spectrophotometric assays.
No interference with refractometric measurement of total protein concentrations was detected with bilirubin concentrations up to 41.5 mg/dL. Biases in refractometric total protein concentrations were detected and were related to the conversion of refractive index values to total protein concentrations.
Hyperbilirubinemia did not interfere with the refractometric estimation of serum total protein concentration. The agreement among total protein concentrations estimated by 3 refractometers was dependent on the method of conversion of refractive index to total protein concentration and was independent of hyperbilirubinemia.
确定高血清胆红素浓度是否会干扰折射法测定血清总蛋白浓度，并评估折射仪测量之间的潜在偏差。
评估研究。
来自2只健康灵缇犬的血清。
将胆红素溶解于0.1M氢氧化钠中，所得溶液与2只禁食犬的血清混合，以达到高达40mg/dL的各种胆红素浓度。使用3台临床折射仪估算折射法总蛋白浓度。使用生化分析仪通过分光光度法测量基于双缩脲法的总蛋白和胆红素浓度。
胆红素浓度高达41.5mg/dL时，未检测到对总蛋白浓度折射法测量的干扰。检测到折射法总蛋白浓度存在偏差，且与折射率值转换为总蛋白浓度有关。
高胆红素血症不干扰血清总蛋白浓度的折射法估算。3台折射仪估算的总蛋白浓度之间的一致性取决于折射率转换为总蛋白浓度的方法，且与高胆红素血症无关。
