Biofuels Unit, DER-CIEMAT, Avda. Complutense 40, 28040 Madrid, Spain.
Department of Chemical, Environmental and Materials Engineering, University of Jaen, Campus Las Lagunillas, 23071 Jaen, Spain.
Bioresour Technol. 2014 Feb;153:101-7. doi: 10.1016/j.biortech.2013.11.079. Epub 2013 Dec 7.
In this work, the effect of phosphoric acid (1% w/w) in steam explosion pretreatment of water extracted olive tree pruning at 175°C and 195°C was evaluated. The objective is to produce ethanol from all sugars (mainly glucose and xylose) contained in the pretreated material. The water insoluble fraction obtained after pretreatment was used as substrate in a simultaneous saccharification and fermentation (SSF) process by a commercial strain of Saccharomyces cerevisiae. The liquid fraction, containing mainly xylose, was detoxified by alkali and ion-exchange resin and then fermented by the xylose fermenting yeast Scheffersomyces stipitis. Ethanol yields reached in a SSF process were close to 80% when using 15% (w/w) substrate consistency and about 70% of theoretical when using prehydrolysates detoxified by ion-exchange resins. Considering sugars recovery and ethanol yields about 160g of ethanol from kg of water extracted olive tree pruning could be obtained.
在这项工作中，评估了磷酸（1%w/w）在 175°C 和 195°C 下蒸汽爆破预处理水提橄榄油修剪物的效果。目的是从预处理材料中所含的所有糖（主要是葡萄糖和木糖）生产乙醇。预处理后获得的水不溶性部分用作商业酿酒酵母 Saccharomyces cerevisiae 同步糖化发酵（SSF）过程的底物。含有主要木糖的液体部分用碱和离子交换树脂进行解毒，然后用木糖发酵酵母 Scheffersomyces stipitis 发酵。当使用 15%（w/w）底物浓度进行 SSF 工艺时，乙醇收率接近 80%，而使用离子交换树脂解毒的预水解产物时，乙醇收率接近 70%理论值。考虑到糖的回收和乙醇的产率，从每公斤水提橄榄油修剪物中可以获得约 160 克乙醇。
