Cardoso Luciana Santos, Barreto Andréia de Souza Rocha, Fernandes Jamille Souza, Oliveira Ricardo Riccio, de Souza Robson da Paixão, Carvalho Edgar M, Araujo Maria Ilma
Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia (UFBA), 4110-160 Salvador-BA, Brazil ; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, UFBA, 40171-115 Salvador-BA, Brazil ; Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq-MCT), Brazil.
Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia (UFBA), 4110-160 Salvador-BA, Brazil.
Clin Dev Immunol. 2013;2013:710647. doi: 10.1155/2013/710647. Epub 2013 Nov 18.
The Th2 immune response in chronic schistosomiasis is associated with the development of periportal fibrosis. However, little is known about the phenotype and activation status of T cells in the process. Objective. To evaluate the profile of T cells in schistosomiasis patients with periportal fibrosis. Methods. It was a cross-sectional study, conducted in the village of Agua Preta, Bahia, Brazil, which included 37 subjects with periportal fibrosis determined by ultrasound. Peripheral blood mononuclear cells were obtained by the Ficcol-hypaque gradient and the frequency of T cells expressing the surface markers CD28, CD69, CD25, and CTLA-4 was determined by flow cytometry. Results. The frequency of CD4(+)CD28(+) T lymphocytes was higher in individuals with moderate to severe fibrosis compared to patients with incipient fibrosis. We did not observe any significant difference in the frequency of CD4(+) T cells expressing CD69 among groups of individuals. There was also no significant difference in the frequency of CD8(+) T cells expressing CD28 or CD69 among the studied groups. Individuals with moderate to severe fibrosis presented a lower frequency of CD8(+) T cells, CD4(+)CD25(high) T cells, and CD4(+)CTLA-4(+) T cells when compared to patients without fibrosis or incipient fibrosis. The frequency of CD4(+)CD25(low) cells did not differ between groups. Conclusion. The high frequency of activated T cells coinciding with a low frequency of putative Treg cells may account for the development of periportal fibrosis in human schistosomiasis.
慢性血吸虫病中的Th2免疫反应与门周纤维化的发展有关。然而，在此过程中T细胞的表型和激活状态却鲜为人知。目的。评估患有门周纤维化的血吸虫病患者的T细胞谱。方法。这是一项横断面研究，在巴西巴伊亚州的阿瓜普雷塔村进行，纳入了37名经超声确定患有门周纤维化的受试者。通过Ficcol-泛影葡胺梯度法获取外周血单个核细胞，并通过流式细胞术测定表达表面标志物CD28、CD69、CD25和CTLA-4的T细胞频率。结果。与早期纤维化患者相比，中度至重度纤维化患者中CD4(+)CD28(+) T淋巴细胞的频率更高。我们未观察到各组个体中表达CD69的CD4(+) T细胞频率有任何显著差异。在所研究的组中，表达CD28或CD69的CD8(+) T细胞频率也没有显著差异。与无纤维化或早期纤维化患者相比，中度至重度纤维化患者的CD8(+) T细胞、CD4(+)CD25(高) T细胞和CD4(+)CTLA-4(+) T细胞频率较低。各组之间CD4(+)CD25(低)细胞的频率没有差异。结论。活化T细胞的高频率与假定的调节性T细胞的低频率同时出现，可能是人类血吸虫病门周纤维化发展的原因。
