• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

15-羟基前列腺素脱氢酶基因对胃癌细胞（小鼠前胃癌）增殖的影响

Effect of 15-hydroxyprostaglandin dehydrogenase gene on the proliferation of gastric cancer cell murine forestomach carcinoma.

作者信息

Li Lihua, Yang Fu, Wang Xiaojie, Hu Jing, Yang Libo, Tang Chunming, Wu Yunhua, Miao Kun, Liu Rui, Shou Tao

机构信息

Department of Oncology, The First Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China.

出版信息

Exp Ther Med. 2014 Jan;7(1):290-294. doi: 10.3892/etm.2013.1404. Epub 2013 Nov 12.

DOI:10.3892/etm.2013.1404
PMID:24348808
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3861248/
Abstract

The aim of the present study was to construct the eukaryotic expression vector pcDNA3.1/15-PGDH. The vector was used to transfect mouse murine forestomach carcinoma (MFC) cancer cells and observe the effects of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) on the proliferation of MFC. pcDNA3.1/15-PGDH was constructed using gene recombination technology and the vector was used to transfect MFC cells to build a stable transfected cell strain. The expression levels of 15-PGDH in the transfected cells were detected using reverse transcription polymerase chain reaction. Optical Density (OD) values were determined using an MTT assay and used to draw cell growth curves. The effects of 15-PGDH on the proliferation of MFC were observed using a clone formation experiment. Following successful transfection by 15-PGDH, the relative expression levels of 15-PGDH in the MFC/15-PGDH cells were significantly higher (1.06±0.08) (P<0.01) compared with the empty plasmid-transfected group (0.22±0.01) and the untransfected group (0.21±0.01). Following transfection by 15-PGDH, cell growth was markedly inhibited. The MTT results showed that on days 4, 6 and 8, the 15-PGDH-transfected group had a low OD on average, which was significantly different (P<0.05) from the empty plasmid-transfected group or the untransfected group. The 15-PGDH-transfected group had a plating efficiency of 18%, and compared with the untransfected group (63%) and the empty plasmid-transfected group (59%), clone formation was significantly inhibited (P<0.01). Results of the present study indicate that transfection by 15-PGDH may significantly inhibit the proliferation and clone formation of MFC cells.

摘要

本研究的目的是构建真核表达载体pcDNA3.1/15-PGDH。该载体用于转染小鼠前胃癌（MFC）癌细胞，观察15-羟基前列腺素脱氢酶（15-PGDH）对MFC细胞增殖的影响。采用基因重组技术构建pcDNA3.1/15-PGDH，并将该载体用于转染MFC细胞以构建稳定转染细胞株。采用逆转录聚合酶链反应检测转染细胞中15-PGDH的表达水平。使用MTT法测定光密度（OD）值并绘制细胞生长曲线。采用克隆形成实验观察15-PGDH对MFC细胞增殖的影响。15-PGDH成功转染后，MFC/15-PGDH细胞中15-PGDH的相对表达水平（1.06±0.08）显著高于空质粒转染组（0.22±0.01）和未转染组（0.21±0.01）（P<0.01）。15-PGDH转染后，细胞生长受到明显抑制。MTT结果显示，在第4、6和8天，15-PGDH转染组的平均OD值较低，与空质粒转染组或未转染组相比有显著差异（P<0.05）。15-PGDH转染组的平板接种效率为18%，与未转染组（63%）和空质粒转染组（59%）相比，克隆形成受到显著抑制（P<0.01）。本研究结果表明，15-PGDH转染可能显著抑制MFC细胞的增殖和克隆形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/8fb6c37836cb/ETM-07-01-0290-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/c361b27e22a0/ETM-07-01-0290-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/98c06caaf79c/ETM-07-01-0290-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/4ca95953d1a8/ETM-07-01-0290-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/737ebae85012/ETM-07-01-0290-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/ea2a36796dc6/ETM-07-01-0290-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/3d03621d18ac/ETM-07-01-0290-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/9971c901a2c4/ETM-07-01-0290-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/8fb6c37836cb/ETM-07-01-0290-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/c361b27e22a0/ETM-07-01-0290-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/98c06caaf79c/ETM-07-01-0290-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/4ca95953d1a8/ETM-07-01-0290-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/737ebae85012/ETM-07-01-0290-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/ea2a36796dc6/ETM-07-01-0290-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/3d03621d18ac/ETM-07-01-0290-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/9971c901a2c4/ETM-07-01-0290-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6799/3861248/8fb6c37836cb/ETM-07-01-0290-g07.jpg

相似文献

1
Effect of 15-hydroxyprostaglandin dehydrogenase gene on the proliferation of gastric cancer cell murine forestomach carcinoma.15-羟基前列腺素脱氢酶基因对胃癌细胞（小鼠前胃癌）增殖的影响
Exp Ther Med. 2014 Jan;7(1):290-294. doi: 10.3892/etm.2013.1404. Epub 2013 Nov 12.
2
15-Hydroxyprostaglandin dehydrogenase is a tumor suppressor of human gastric cancer.15-羟基前列腺素脱氢酶是人胃肿瘤的一种肿瘤抑制因子。
Cancer Biol Ther. 2010 Oct 15;10(8):780-7. doi: 10.4161/cbt.10.8.12896.
3
15-PGDH is reduced and induces apoptosis and cell cycle arrest in gastric carcinoma.15-PGDH 减少并诱导胃癌细胞凋亡和细胞周期停滞。
World J Gastroenterol. 2012 Mar 14;18(10):1028-37. doi: 10.3748/wjg.v18.i10.1028.
4
Suppression of invasive properties of colorectal carcinoma SW480 cells by 15-hydroxyprostaglandin dehydrogenase gene.15-羟基前列腺素脱氢酶基因对结肠直肠癌SW480细胞侵袭特性的抑制作用
Cancer Invest. 2008 Nov;26(9):905-12. doi: 10.1080/07357900802146154.
5
15-hydroxyprostaglandin dehydrogenase is downregulated and exhibits tumor suppressor activity in gastric cancer.15-羟基前列腺素脱氢酶在胃癌中下调并表现出肿瘤抑制活性。
Cancer Invest. 2011 May;29(4):257-65. doi: 10.3109/07357907.2011.568562.
6
Role of 15-hydroxyprostaglandin dehydrogenase down-regulation on the prognosis of hepatocellular carcinoma.15-羟基前列腺素脱氢酶下调在肝细胞癌预后中的作用。
Clin Mol Hepatol. 2014 Mar;20(1):28-37. doi: 10.3350/cmh.2014.20.1.28. Epub 2014 Mar 26.
7
15-hydroxyprostaglandin dehydrogenase is down-regulated in gastric cancer.15-羟基前列腺素脱氢酶在胃癌中表达下调。
Clin Cancer Res. 2009 Jul 15;15(14):4572-80. doi: 10.1158/1078-0432.CCR-08-2518. Epub 2009 Jul 7.
8
Expression of 15-PGDH is downregulated by COX-2 in gastric cancer.在胃癌中，15-前列腺素脱氢酶（15-PGDH）的表达受环氧合酶-2（COX-2）下调。
Carcinogenesis. 2008 Jun;29(6):1219-27. doi: 10.1093/carcin/bgm297. Epub 2008 Jan 3.
9
[Impact of the biological function on epithelial ovarian cancer with ITIH4 gene expression down-regulating in vitro].[ITIH4基因表达下调对体外培养的上皮性卵巢癌生物学功能的影响]
Zhonghua Fu Chan Ke Za Zhi. 2013 Jan;48(1):34-40.
10
15-hydroxyprostaglandin dehydrogenase is a tumor suppressor of human breast cancer.15-羟基前列腺素脱氢酶是人类乳腺癌的一种肿瘤抑制因子。
Cancer Res. 2006 Aug 1;66(15):7818-23. doi: 10.1158/0008-5472.CAN-05-4368.

引用本文的文献

1
CD45 erythroid progenitor cells promote lymph node metastasis in gastric cancer by inducing a hybrid epithelial/mesenchymal state in lymphatic endothelial cells.CD45 红细胞祖细胞通过诱导淋巴管内皮细胞的混合上皮/间充质状态促进胃癌淋巴结转移。
Gastric Cancer. 2023 Nov;26(6):918-933. doi: 10.1007/s10120-023-01425-x. Epub 2023 Sep 7.
2
MicroRNA-21 induces loss of 15-hydroxyprostaglandin dehydrogenase in early gastric tubular adenocarcinoma.微小 RNA-21 诱导早期胃管状腺癌中 15-羟基前列腺素脱氢酶的缺失。
Sci Rep. 2018 Dec 7;8(1):17717. doi: 10.1038/s41598-018-36139-z.
3
MicroRNAs: promising chemoresistance biomarkers in gastric cancer with diagnostic and therapeutic potential.

本文引用的文献

1
15-PGDH is reduced and induces apoptosis and cell cycle arrest in gastric carcinoma.15-PGDH 减少并诱导胃癌细胞凋亡和细胞周期停滞。
World J Gastroenterol. 2012 Mar 14;18(10):1028-37. doi: 10.3748/wjg.v18.i10.1028.
2
Colon tumour cells increase PGE(2) by regulating COX-2 and 15-PGDH to promote survival during the microenvironmental stress of glucose deprivation.结肠肿瘤细胞通过调节 COX-2 和 15-PGDH 增加 PGE(2)，以促进在葡萄糖剥夺的微环境应激下的存活。
Carcinogenesis. 2011 Nov;32(11):1741-7. doi: 10.1093/carcin/bgr210. Epub 2011 Sep 16.
3
15-Hydroxyprostaglandin dehydrogenase as a novel molecular target for cancer chemoprevention and therapy.
微小RNA：胃癌中具有诊断和治疗潜力的有前景的化疗耐药生物标志物。
World J Gastroenterol. 2014 Oct 14;20(38):13658-66. doi: 10.3748/wjg.v20.i38.13658.
15-羟基前列腺素脱氢酶作为癌症化学预防和治疗的新分子靶标。
Biochem Pharmacol. 2011 Nov 15;82(10):1352-60. doi: 10.1016/j.bcp.2011.08.005. Epub 2011 Aug 11.
4
15-hydroxyprostaglandin dehydrogenase is downregulated and exhibits tumor suppressor activity in gastric cancer.15-羟基前列腺素脱氢酶在胃癌中下调并表现出肿瘤抑制活性。
Cancer Invest. 2011 May;29(4):257-65. doi: 10.3109/07357907.2011.568562.
5
Loss of 15-hydroxyprostaglandin dehydrogenase expression contributes to bladder cancer progression.15-羟基前列腺素脱氢酶表达缺失促进膀胱癌进展。
Am J Pathol. 2010 Mar;176(3):1462-8. doi: 10.2353/ajpath.2010.090875. Epub 2010 Jan 21.
6
Cyclooxygenase-2 expression is related to the epithelial-to-mesenchymal transition in human colon cancers.环氧合酶-2 的表达与人类结肠癌的上皮-间充质转化有关。
Yonsei Med J. 2009 Dec 31;50(6):818-24. doi: 10.3349/ymj.2009.50.6.818. Epub 2009 Dec 18.
7
Experimental cancer therapy using restoration of NAD+ -linked 15-hydroxyprostaglandin dehydrogenase expression.利用恢复 NAD+ 连接的 15-羟基前列腺素脱氢酶表达进行癌症治疗的实验。
Mol Cancer Ther. 2009 Nov;8(11):3130-9. doi: 10.1158/1535-7163.MCT-09-0270. Epub 2009 Nov 3.
8
HGF/Met signalling promotes PGE(2) biogenesis via regulation of COX-2 and 15-PGDH expression in colorectal cancer cells.HGF/Met信号通路通过调节结肠癌细胞中COX-2和15-PGDH的表达促进前列腺素E2（PGE2）的生物合成。
Carcinogenesis. 2009 Oct;30(10):1796-804. doi: 10.1093/carcin/bgp183. Epub 2009 Jul 28.
9
15-hydroxyprostaglandin dehydrogenase is down-regulated in gastric cancer.15-羟基前列腺素脱氢酶在胃癌中表达下调。
Clin Cancer Res. 2009 Jul 15;15(14):4572-80. doi: 10.1158/1078-0432.CCR-08-2518. Epub 2009 Jul 7.
10
Blockade of cholecystokinin-2 receptor and cyclooxygenase-2 synergistically induces cell apoptosis, and inhibits the proliferation of human gastric cancer cells in vitro.胆囊收缩素-2受体和环氧化酶-2的阻断协同诱导细胞凋亡，并在体外抑制人胃癌细胞的增殖。
Cancer Lett. 2008 May 18;263(2):302-11. doi: 10.1016/j.canlet.2008.01.012. Epub 2008 Feb 6.