兔肌肉1,4-α-葡聚糖分支酶核酸组分的催化活性
Catalytic activity of the nucleic acid component of the 1,4-alpha-glucan branching enzyme from rabbit muscles.
作者信息
Shvedova T A, Korneeva G A, Otroshchenko V A, Venkstern T V
出版信息
Nucleic Acids Res. 1987 Feb 25;15(4):1745-52. doi: 10.1093/nar/15.4.1745.
Abstract
2.5 S RNA, the nucleic acid component of the 1,4-alpha-D-glucan: 1,4-alpha-D-glucan 6-alpha-(1,4-alpha-glucano)-transferase from rabbit muscles, devoid of any protein, catalyses the branching reaction, as does the holoenzyme. The conclusion is drawn that 2.5 S RNA is a ribozyme. To get an insight into the significance of different parts of the molecule for the catalytic activity of 2.5 S RNA, a large fragment isolated from its partial RNAase A digest was investigated. This fragment which proved to be the middle part of polyribonucleotide chain containing all modified nucleotides exerts some catalytic activity, too.
摘要
2.5S RNA，即来自兔肌肉的1,4-α-D-葡聚糖：1,4-α-D-葡聚糖6-α-(1,4-α-葡聚糖基)-转移酶的核酸成分，在没有任何蛋白质的情况下，与全酶一样催化分支反应。由此得出结论，2.5S RNA是一种核酶。为了深入了解分子不同部分对2.5S RNA催化活性的重要性，对从其部分核糖核酸酶A消化产物中分离出的一个大片段进行了研究。这个片段被证明是多核糖核苷酸链的中间部分，包含所有修饰核苷酸，它也具有一定的催化活性。
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本文引用的文献
1
[Studies on active forms of amylose isomerase].
Biokhimiia. 1961 Nov-Dec;26:1001-7.
2
Specific interaction between the self-splicing RNA of Tetrahymena and its guanosine substrate: implications for biological catalysis by RNA.嗜热四膜虫自我剪接RNA与其鸟苷底物之间的特异性相互作用：对RNA生物催化的启示
Nature. 1984;308(5962):820-6. doi: 10.1038/308820a0.
3
[Structure-function study of 1,4-alpha-glucan branching enzyme by limited trypsin treatment].
Mol Biol (Mosk). 1982 Jul-Aug;16(4):731-8.
4
Catalytic activity of an RNA molecule prepared by transcription in vitro.通过体外转录制备的RNA分子的催化活性。
Science. 1984 Jan 20;223(4633):285-6. doi: 10.1126/science.6199841.
5
First true RNA catalyst found.首个真正的RNA催化剂被发现。
Science. 1984 Jan 20;223(4633):266-7. doi: 10.1126/science.6199840.
6
[Study of 2.5S RNA of 1,4-alpha-glucan branching enzyme by fluorescent methods using ethidium bromide].[利用溴化乙锭荧光法对1,4-α-葡聚糖分支酶的2.5S RNA进行的研究]
Mol Biol (Mosk). 1983 Nov-Dec;17(6):1186-95.
7
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme.核糖核酸酶P的RNA部分是该酶的催化亚基。
Cell. 1983 Dec;35(3 Pt 2):849-57. doi: 10.1016/0092-8674(83)90117-4.
8
In vitro splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence.嗜热四膜虫核糖体RNA前体的体外剪接：鸟苷酸在间隔序列切除中的作用。
Cell. 1981 Dec;27(3 Pt 2):487-96. doi: 10.1016/0092-8674(81)90390-1.
9
On the participation of lowpolymeric (3S) ribonucleic acid in the branching reaction of polysaccharides.关于低聚（3S）核糖核酸在多糖分支反应中的参与情况。
Enzymologia. 1971 Feb 28;40(2):127-39.
10
[Reconstruction of the protein components of the muscle branching enzyme--amylose isomerase].[肌肉分支酶——直链淀粉异构酶蛋白质成分的重建]
Dokl Akad Nauk SSSR. 1974;218(5):1242-4.
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