Abreu-Velez Ana Maria, Googe Paul B, Howard Michael S
Georgia Dermatopathology Associates, Atlanta, Georgia.
Knoxville Dermatopathology Laboratory, Knoxville, Tennessee, USA.
N Am J Med Sci. 2013 Oct;5(10):604-8. doi: 10.4103/1947-2714.120797.
The in situ signaling transduction within skin biopsies from patients affected by autoimmune skin blistering diseases is not well-characterized.
In autoimmune skin blistering diseases, autoantibodies seem to trigger several intracellular signaling pathways and we investigated the presence of the phosphorylated form of ribosomal protein S6-pS240 within autoimmune skin blistering diseases biopsies.
We utilized immunohistochemistry to evaluate the presence of S6-pS240 in lesional skin biopsies of patients affected by autoimmune skin blistering diseases including patients with an endemic and nonendemic pemphigus foliaceus (non EPF), with bullous pemphigoid (BP), pemphigus vulgaris (PV), dermatitis herpetiformis (DH), and the respective controls.
Most autoimmune bullous skin diseases biopsies stained positive for S6-pS240 around lesional blisters, including adjacent areas of the epidermis; and within upper dermal inflammatory infiltrates, and/or mesenchymal-endothelial cell junctions within the dermis.
We document that S6-pS240 is expressed in lesional areas of skin biopsies from patients with autoimmune skin blistering diseases, as well as on eccrine glands and piloerector muscles. Thus, the role of this molecule in autoimmune skin blistering diseases warrants further study.
自身免疫性皮肤水疱病患者皮肤活检组织内的原位信号转导尚未得到充分表征。
在自身免疫性皮肤水疱病中，自身抗体似乎会触发多种细胞内信号通路，我们研究了自身免疫性皮肤水疱病活检组织中核糖体蛋白S6 - pS240磷酸化形式的存在情况。
我们利用免疫组织化学方法评估了S6 - pS240在自身免疫性皮肤水疱病患者皮损皮肤活检组织中的存在情况，这些患者包括地方性和非地方性落叶型天疱疮（非EPF）、大疱性类天疱疮（BP）、寻常型天疱疮（PV）、疱疹样皮炎（DH）患者以及相应的对照。
大多数自身免疫性大疱性皮肤病活检组织在皮损水疱周围，包括表皮的相邻区域；真皮上部炎症浸润内，和/或真皮内的间充质 - 内皮细胞连接处，S6 - pS240染色呈阳性。
我们证明S6 - pS240在自身免疫性皮肤水疱病患者皮肤活检组织的皮损区域以及汗腺和竖毛肌中表达。因此，该分子在自身免疫性皮肤水疱病中的作用值得进一步研究。
