Landman G, Correa Alves A, Mendes N F, Mendes E
Allergol Immunopathol (Madr). 1986 Nov-Dec;14(6):509-13.
The immunoperoxidase technique using a polyclonal T. cruzi rabbit antiserum was compared with the routine hematoxylin-eosin staining procedure in the study of two cases of congenital Chagas disease. Comparatively, the immunoperoxidase technique gave better results than that using hematoxylin-eosin in identification of T. cruzi. The immunoperoxidase technique detected T. cruzi nests in unsuspected sites like lungs and testes, in which the hematoxylin-eosin stain failed to demonstrate their presence. In the two cases studied there was no evidence of positive immunoperoxidase reaction which could suggest the presence of cross-antigens between T. cruzi and host tissues, in the heart, gastrointestinal tract or other organs. These negative results are discussed.
在两例先天性恰加斯病的研究中，将使用多克隆克氏锥虫兔抗血清的免疫过氧化物酶技术与常规苏木精 - 伊红染色程序进行了比较。相比之下，在克氏锥虫的鉴定方面，免疫过氧化物酶技术比苏木精 - 伊红染色效果更好。免疫过氧化物酶技术在肺和睾丸等未被怀疑的部位检测到了克氏锥虫巢，而苏木精 - 伊红染色未能显示它们的存在。在所研究的两例病例中，在心脏、胃肠道或其他器官中，没有证据表明免疫过氧化物酶反应呈阳性，提示克氏锥虫与宿主组织之间存在交叉抗原。对这些阴性结果进行了讨论。
