冷暴露大鼠脱适应后棕色脂肪组织解偶联蛋白mRNA的丧失
Loss of brown adipose tissue uncoupling protein mRNA on deacclimation of cold-exposed rats.
作者信息
Reichling S, Ridley R G, Patel H V, Harley C B, Freeman K B
出版信息
Biochem Biophys Res Commun. 1987 Feb 13;142(3):696-701. doi: 10.1016/0006-291x(87)91470-7.
The effect of environmental temperature on the level of uncoupling protein mRNA from rat brown adipose tissue was examined using a cDNA probe. A 4.4 fold increase in the mRNA level was observed after 1 day exposure of rats to 6 degrees C, which was followed by a slow loss with longer times of exposure. When rats were returned to a thermoneutral environment, there was a dramatic loss of uncoupling protein mRNA within 1 day. Comparison wih poly(A)+ RNA levels suggest that the response to temperature is specific for uncoupling protein mRNA.
使用cDNA探针检测了环境温度对大鼠棕色脂肪组织解偶联蛋白mRNA水平的影响。将大鼠暴露于6摄氏度环境1天后，观察到mRNA水平增加了4.4倍，随后随着暴露时间延长而缓慢下降。当大鼠回到热中性环境时，1天内解偶联蛋白mRNA显著减少。与聚腺苷酸加尾RNA水平的比较表明，对温度的反应是解偶联蛋白mRNA所特有的。
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