Zheng Liqin, Chen Jiangxu, Huang Yimei, Wang Yuhua, Yang Hongqin, Zhang Yanding, Xie Shusen
Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Collage of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou, 350007, China.
Lasers Med Sci. 2014 May;29(3):1209-15. doi: 10.1007/s10103-013-1511-2. Epub 2013 Dec 19.
Adenosine exerts a key role in analgesia. In the present study, adenosine-induced Ca(2+) responses were revealed by using confocal microscopy imaging in the rat dorsal root ganglia (DRG) neurons in vitro. Our results showed that adenosine could evoke increases in the intracellular Ca(2+) concentration in the DRG neurons. In addition, by application of selective receptor antagonists, two types of receptors, A1R and A3R, were identified to be involved in the adenosine-induced Ca(2+) release from intracellular stores in neurons. Altogether, these results suggest that confocal microscopy imaging combined with fluorescent dyes could help to detect the analgesic-induced ion signaling in single cell.
腺苷在镇痛中发挥关键作用。在本研究中，通过共聚焦显微镜成像在体外大鼠背根神经节（DRG）神经元中揭示了腺苷诱导的Ca(2+)反应。我们的结果表明，腺苷可引起DRG神经元细胞内Ca(2+)浓度升高。此外，通过应用选择性受体拮抗剂，确定了两种受体，即A1R和A3R，参与了腺苷诱导的神经元细胞内储存Ca(2+)释放。总之，这些结果表明，共聚焦显微镜成像与荧光染料相结合有助于检测单细胞中镇痛诱导的离子信号。
