大鼠骨髓间充质干细胞促进大鼠薄型子宫内膜再生。

Rat bone marrow mesenchymal stem cells improve regeneration of thin endometrium in rat.

机构信息

Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

出版信息

Fertil Steril. 2014 Feb;101(2):587-94. doi: 10.1016/j.fertnstert.2013.10.053. Epub 2013 Dec 17.

DOI:10.1016/j.fertnstert.2013.10.053

PMID:24355044

Abstract

OBJECTIVE

To investigate whether bone marrow mesenchymal stem cell (BMSC) treatment could improve the regeneration of endometrium and improve the endometrial receptivity in an experimental model of thin endometrium.

DESIGN

Randomized, control trial, animal research.

SETTING

National key laboratory.

ANIMAL(S): Sprague-Dawley rats.

INTERVENTION(S): Bone marrow mesenchymal stem cell transplantation by tail vein IV injection.

MAIN OUTCOME MEASURE(S): Endometrial thickness, the expression of mark proteins for endometrial cell, and endometrial receptivity.

RESULT(S): The endometrium was significantly thicker and the expression of cytokeratin, vimentin, integrin αγβ3, and leukemia inhibitor factor were significantly stronger compared with the control group. Some proinflammatory cytokines, such as tumor necrosis factor-α (TNF-α) messenger RNA (mRNA) and interleukin-1β mRNA, were significantly down-regulated, and anti-inflammatory cytokines, such as fibroblast growth factor-β (bFGF) mRNA and interleukin-6 mRNA, were significantly up-regulated in the experimental group compared with the control group.

CONCLUSION(S): The BMSCs have beneficial effect on thin endometrium, and may play a role through migration and immunomodulatory of BMSCs.

摘要

目的

研究骨髓间充质干细胞（BMSC）治疗能否改善薄型子宫内膜的内膜再生和提高内膜容受性。

设计

随机对照试验，动物研究。

地点

国家重点实验室。

动物

Sprague-Dawley 大鼠。

干预

通过尾静脉 IV 注射进行骨髓间充质干细胞移植。

主要观察指标

子宫内膜厚度、子宫内膜细胞标志物的表达和子宫内膜容受性。

结果

与对照组相比，子宫内膜明显增厚，角蛋白、波形蛋白、整合素 αγβ3 和白血病抑制因子的表达明显增强。实验组中一些促炎细胞因子，如肿瘤坏死因子-α（TNF-α）信使 RNA（mRNA）和白细胞介素-1β mRNA 的表达明显下调，而抗炎细胞因子，如成纤维细胞生长因子-β（bFGF）mRNA 和白细胞介素-6 mRNA 的表达明显上调。