Fish Biology Research Unit, Department of Zoology, School of Life Sciences, Visva-Bharati, A Central University, Santiniketan 731235, West Bengal, India.
Department of Zoology, Maulana Azad College, 8 Rafi Ahmed Kidawi Road, Kolkata 700013, West Bengal, India.
Tissue Cell. 2014 Feb;46(1):59-69. doi: 10.1016/j.tice.2013.11.003. Epub 2013 Nov 17.
The present study has been carried out to describe the cell morphology of the developing male gametes in a fish ectoparasite, Argulus bengalensis Ramakrishna, 1951. With respect to cell volume and nucleoplasmic index, spermatogonia are the smallest and primary spermatocytes are the largest in this lineage. The spermatogonia and the differentiating spermatogenic cells are in separate niches and confined to different enclaves within each testicular lobe. Spermiogenesis occurs within the inner enclave of each testicular lobe. During this process the nucleus becomes streamlined; an acrosome is formed, axoneme is originated, and residual cytoplasm is discarded through the flagellum. The sperm cell morphology displays a general pattern comprising head, mid-piece, and a full length flagellum. In the axoneme 9+2 arrangement of the microtubule is conserved. In addition to the axoneme, some more singlet microtubules are found surrounding a fiber sheath and around one of the mitochondria adjacent to the axoneme. This arrangement indicates a close phylogenetic relationship with pentastomida. In the present study, structure and formation of spermatophore are described in this branchiuran parasite.
本研究旨在描述鱼类外寄生虫印度沼虾 Argulus bengalensis Ramakrishna, 1951 中雄性配子的细胞形态。就细胞体积和核质指数而言，精原细胞是最小的，初级精母细胞是最大的。精原细胞和分化的精母细胞位于不同的龛位，局限于每个精巢叶的不同腔内。精子发生发生在每个精巢叶的内腔内。在此过程中，核变得流线型；顶体形成，轴丝起源，剩余的细胞质通过鞭毛被丢弃。精子细胞形态显示出一种包含头部、中段和全长鞭毛的一般模式。在轴丝中，微管的 9+2 排列得以保留。除了轴丝外，还发现一些单独的微管围绕纤维鞘和靠近轴丝的一个线粒体周围。这种排列表明与 pentastomida 具有密切的系统发育关系。在本研究中，描述了这种鳃足类寄生虫的精荚的结构和形成。
