Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China.
Anal Chim Acta. 2014 Jan 7;807:126-34. doi: 10.1016/j.aca.2013.11.042. Epub 2013 Dec 1.
Using the Hg(2+)-induced desulfurization reaction of thiosemicarbazide derivative, we designed and synthesized a novel "turn on" coumarin-based fluorescent probe L with a simple structure for detecting mercury ion (II). Spectroscopy revealed that the probe responds selectively to mercury ions over other metal ions with marked fluorescence enhancement. Detection of Hg(2+) was effective at pH 7.0-9.5, with high selectivity and significant effect in HeLa cells, human umbilical vein endothelial cells and Escherichia coli, but no cytotoxicity. This probe could be an ideal and practical Hg(2+) probe with important biological significance.
利用汞离子（Hg(2+)）诱导的缩氨基硫脲衍生物的脱硫反应，我们设计并合成了一种新型的基于香豆素的荧光探针 L，其结构简单，可用于检测汞离子（II）。光谱研究表明，该探针对汞离子具有选择性响应，并且在其他金属离子存在下，荧光显著增强。在 pH 值为 7.0-9.5 时，对 Hg(2+)的检测效果良好，具有高选择性和明显的效果，在 HeLa 细胞、人脐静脉内皮细胞和大肠杆菌中均有应用，但无细胞毒性。该探针可能是一种具有重要生物学意义的理想实用 Hg(2+)探针。
