Azurmendi Pablo J, Oddo Elisabet M, Toledo Jorge E, Martin Rodolfo S, Ibarra Fernando R, Arrizurieta Elvira E
Laboratorio de Nefrología Experimental, Instituto de Investigaciones Médicas Alfredo Lanari, Universidad de Buenos Aires.
Medicina (B Aires). 2013;73(6):513-9.
The role played by sexual hormones and vasoactive substances in the compensatory renal growth (CRG) that follows uninephrectomy (uNx) is still controversial. Intact and gonadectomized adult Wistar rats of both sexes, with and without uNx, performed at 90 days age, were studied at age 150 days. Daily urine volume, electrolyte excretion and kallikrein activity (UKa) were determined. Afterwards, glomerular filtration rate and blood pressure were measured, the kidneys weighed and DNA, protein and RNA studied to determine nuclei content and cell size. When the remnant kidney weight at age 150 days was compared with the weight of the kidney removed at the time of uNx, male uNx rats showed the greatest CRG (50%) while growth in the other uNx groups was 25%, 15% and 19% in orchidectomized, female and ovariectomized rats, respectively. The small CRG observed in the uNx female rats was accompanied by the lowest glomerular filtration value, 0.56 ± 0.02 ml/ min/g kwt compared, with the other uNx groups, p < 0.05. Cell size (protein or RNA/DNA) was similar for all the groups except for uNx orchidectomized rats. In this group the cytoplasmatic protein or RNA content was lower than in the other groups while DNA (nuclei content) was similar. Some degree of hyperplasia was determined by DNA content in the uNx groups. Male sexual hormones positively influenced CRG and its absence modulated cell size. Female sexual hormones, instead, did not appear to stimulate CRG. The kallikrein kinin system may not be involved in CRG.
性激素和血管活性物质在单侧肾切除术后的代偿性肾生长（CRG）中所起的作用仍存在争议。对90日龄时进行或未进行单侧肾切除的成年雌雄Wistar大鼠，在150日龄时进行研究，这些大鼠分为完整组和去性腺组。测定每日尿量、电解质排泄和激肽释放酶活性（尿激肽释放酶活性）。随后，测量肾小球滤过率和血压，称量肾脏重量，并研究DNA、蛋白质和RNA以确定细胞核含量和细胞大小。当将150日龄时残余肾脏的重量与单侧肾切除时切除的肾脏重量进行比较时，雄性单侧肾切除大鼠显示出最大的CRG（50%），而在其他单侧肾切除组中，去势雄性大鼠、雌性大鼠和去势雌性大鼠的生长率分别为25%、15%和19%。在单侧肾切除的雌性大鼠中观察到的较小的CRG伴随着最低的肾小球滤过值，与其他单侧肾切除组相比为0.56±0.02 ml/ min/g肾重，p<0.05。除单侧肾切除的去势大鼠组外，所有组的细胞大小（蛋白质或RNA/DNA）相似。在该组中，细胞质蛋白质或RNA含量低于其他组，而DNA（细胞核含量）相似。通过单侧肾切除组中的DNA含量确定了一定程度的增生。雄性性激素对CRG有积极影响，其缺失则调节细胞大小。相反，雌性性激素似乎并未刺激CRG。激肽释放酶-激肽系统可能不参与CRG。
