Gainer Christian F, Joshua Gihan S, Romanowski Marek
Department of Biomedical Engineering, University of Arizona, 1657 E Helen St, Tucson, AZ USA 85721.
Proc SPIE Int Soc Opt Eng. 2012 Feb 1;8231:82310I-. doi: 10.1117/12.909343.
In the interest of generating new biomedical sensing techniques as well as improving those that currently exist, a great deal of attention has been given to upconverting lanthanide nanoparticles in recent years. In order to develop these nanoparticles for use in multiplexed and ratiometric sensing techniques, many recent studies have focused on experimental control of their emission wavelengths. Here we describe a new method for controlling the relative intensity of green and red emission bands in NaYF:Yb, Er nanoparticles via control of the excitation pulse repetition rate. Using this method, particles of the same composition may be tuned to produce red and green light in user-defined ratios. We discuss the mechanism behind this control as well as potential applications that could make use of this property, specifically in super resolution imaging techniques.
为了开发新的生物医学传感技术并改进现有技术，近年来，上转换镧系纳米粒子受到了广泛关注。为了将这些纳米粒子用于多路复用和比率传感技术，最近的许多研究都集中在对其发射波长的实验控制上。在此，我们描述了一种通过控制激发脉冲重复率来控制NaYF:Yb, Er纳米粒子中绿色和红色发射带相对强度的新方法。使用这种方法，可以将相同组成的粒子调整为以用户定义的比例产生红光和绿光。我们讨论了这种控制背后的机制以及可以利用此特性的潜在应用，特别是在超分辨率成像技术中。
