基因转换变异在淋病奈瑟菌中产生结构不同的菌毛多肽。
Gene conversion variations generate structurally distinct pilin polypeptides in Neisseria gonorrhoeae.
作者信息
Swanson J, Robbins K, Barrera O, Koomey J M
出版信息
J Exp Med. 1987 Apr 1;165(4):1016-25. doi: 10.1084/jem.165.4.1016.
Abstract
Pilus+ to pilus- phenotype change occurs in Neisseria gonorrhoeae through gene conversion of the gonococcus' complete, expressed pilin gene by nucleotides homologous to the pilS1 copy 5 partial pilin gene; assembly missense pilin is synthesized but pili are not. Reversion to pilus+ occurs by a subsequent recombinational event that replaces the complete pilin gene's pilS1 copy 5-like sequence with nucleotides from a different partial gene to effect expression of an orthodox (i.e., pilus producing) pilin. Sibling pilus+ revertants of common parentage can carry different sequences in their expressed pilin genes because they have undergone nonidentical gene conversion events such as recombinations with sequences from different partial genes, or recombinations with different length nucleotide stretches of the same partial gene; either can yield structurally and antigenically variant pilin polypeptides.
摘要
淋病奈瑟菌中菌毛阳性到菌毛阴性的表型变化是通过淋球菌完整的、表达的菌毛蛋白基因被与pilS1拷贝5部分菌毛蛋白基因同源的核苷酸进行基因转换而发生的；组装错义菌毛蛋白被合成，但菌毛未形成。通过随后的重组事件可恢复为菌毛阳性，该重组事件用来自不同部分基因的核苷酸取代完整菌毛蛋白基因的pilS1拷贝5样序列，以实现正统（即产生菌毛的）菌毛蛋白的表达。具有共同亲本的同胞菌毛阳性回复株在其表达的菌毛蛋白基因中可携带不同序列，因为它们经历了不同的基因转换事件，例如与来自不同部分基因的序列重组，或与同一部分基因的不同长度核苷酸片段重组；两者都可产生结构和抗原性不同的菌毛蛋白多肽。
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Antigenic analysis of gonococcal pili using monoclonal antibodies.使用单克隆抗体对淋球菌菌毛进行抗原分析。
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Gene conversion involving the pilin structural gene correlates with pilus+ in equilibrium with pilus- changes in Neisseria gonorrhoeae.涉及菌毛蛋白结构基因的基因转换与淋病奈瑟菌中菌毛阳性与菌毛阴性平衡变化相关。
Cell. 1986 Oct 24;47(2):267-76. doi: 10.1016/0092-8674(86)90449-6.
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Proc Natl Acad Sci U S A. 1986 Jun;83(11):3890-4. doi: 10.1073/pnas.83.11.3890.
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The repertoire of silent pilus genes in Neisseria gonorrhoeae: evidence for gene conversion.淋病奈瑟菌中沉默菌毛基因的库：基因转换的证据。
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Intragenic recombination leads to pilus antigenic variation in Neisseria gonorrhoeae.基因内重组导致淋病奈瑟菌菌毛抗原变异。
Nature. 1985;315(6015):156-8. doi: 10.1038/315156a0.
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Pilus- gonococcal variants. Evidence for multiple forms of piliation control.菌毛 - 淋球菌变体。多种菌毛调控形式的证据。
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