Harry G J, Goodrum J F, Toews A D, Morell P
J Neurochem. 1987 May;48(5):1529-36. doi: 10.1111/j.1471-4159.1987.tb05696.x.
The distribution of axonally transported gangliosides and glycoproteins along the sciatic nerve was examined from 3 h to 4 weeks following injection of[3H]glucosamine into the fifth lumbar dorsal root ganglion of adult rats. Incorporation of labeled precursor into these glycoconjugates reached a maximal level in the ganglion within 6 h. Outflow patterns of radioactivity for glycoproteins showed a well-defined crest with a transport rate of approximately 330 mm/day. In contrast, the crest of transported gangliosides was continuously attenuated, implying a significant deposition along the axon, and an alternative method of calculating velocity was required. Analysis of accumulation of labeled material at double ligatures demonstrated both anterograde and retrograde transport of glycoproteins and gangliosides and allowed for the calculation of an anterograde transport rate of about 270 mm/day for each. Additional evidence of ganglioside transport is provided in that the TLC pattern of transported radioactive gangliosides accumulating at a ligature is significantly different from the pattern seen in the dorsal root ganglion or following intraneural administration of the labeled precursor. These data indicate that gangliosides are transported at the same rapid rate as glycoproteins but are subject to a more extensive exchange with stationary material than are glycoproteins.
在成年大鼠第五腰段背根神经节注射[3H]葡糖胺后3小时至4周，研究了轴突运输的神经节苷脂和糖蛋白沿坐骨神经的分布情况。标记前体掺入这些糖缀合物的过程在6小时内在神经节内达到最大水平。糖蛋白的放射性流出模式显示出一个明确的峰值，运输速率约为330毫米/天。相比之下，运输的神经节苷脂的峰值持续衰减，这意味着沿轴突有大量沉积，因此需要一种计算速度的替代方法。对双结扎处标记物质积累的分析表明，糖蛋白和神经节苷脂既有顺行运输也有逆行运输，并且可以计算出每种物质的顺行运输速率约为270毫米/天。神经节苷脂运输的额外证据在于，在结扎处积累的运输放射性神经节苷脂的薄层色谱模式与在背根神经节中看到的模式或在神经内注射标记前体后看到的模式有显著差异。这些数据表明，神经节苷脂与糖蛋白以相同的快速速率运输，但与糖蛋白相比，其与固定物质的交换更为广泛。
