Xu J-Y, Zhu W-J, Xie B-G
Institute of Reproductive Immunology, College of Life Science and Technology, Jinan University , Guangzhou , People's Republic of China.
J Obstet Gynaecol. 2014 Jan;34(1):17-20. doi: 10.3109/01443615.2013.822482.
Genetic mutations in the von Hippel-Lindau (VHL) gene are common in certain diseases. The effects on the VHL gene in the tubal pregnancy tissues are unknown but with further study, it was found that the VHL gene may be related to prognosis or therapy selection. This study was conducted to analyse the VHL gene in tissues of human fallopian tube and tubal pregnancy. A total of 35 patients undergoing salpingectomy for tubal pregnancy were recruited into the experimental group. Samples of ampullary fallopian tube during mid-secretory phase were collected from 10 patients with benign uterine disease as the control group. Fluorescent dye dideoxy termination method was performed to detect three exons sequences of the VHL gene in tissues of both the human fallopian tube and tubal pregnancy. The DNA sequences of three exons of VHL gene coding region in tubal pregnancy were not found in mutations. The present study suggested that the VHL gene mutations were not related with tubal pregnancy.
冯·希佩尔-林道（VHL）基因的基因突变在某些疾病中很常见。输卵管妊娠组织中VHL基因所受影响尚不清楚，但通过进一步研究发现，VHL基因可能与预后或治疗选择有关。本研究旨在分析人输卵管及输卵管妊娠组织中的VHL基因。总共35例因输卵管妊娠接受输卵管切除术的患者被纳入实验组。从10例患有良性子宫疾病的患者中采集分泌中期壶腹部输卵管样本作为对照组。采用荧光染料双脱氧末端终止法检测人输卵管及输卵管妊娠组织中VHL基因的三个外显子序列。未在输卵管妊娠中发现VHL基因编码区三个外显子的DNA序列存在突变。本研究表明，VHL基因突变与输卵管妊娠无关。
