时间分辨表面增强红外吸收光谱法研究直接电子转移到 R. sphaeroides 细胞色素 c 氧化酶。
Time-resolved surface-enhanced IR-absorption spectroscopy of direct electron transfer to cytochrome c oxidase from R. sphaeroides.
机构信息
Austrian Institute of Technology, Vienna, Austria.
Austrian Institute of Technology, Vienna, Austria; Center of Electrochemical Surface Technology, Wiener Neustadt, Austria.
出版信息
Biophys J. 2013 Dec 17;105(12):2706-13. doi: 10.1016/j.bpj.2013.10.037.
Abstract
Time-resolved surface-enhanced IR-absorption spectroscopy triggered by electrochemical modulation has been performed on cytochrome c oxidase from Rhodobacter sphaeroides. Single bands isolated from a broad band in the amide I region using phase-sensitive detection were attributed to different redox centers. Their absorbances changing on the millisecond timescale could be fitted to a model based on protonation-dependent chemical reaction kinetics established previously. Substantial conformational changes of secondary structures coupled to redox transitions were revealed.
摘要
时间分辨的表面增强红外吸收光谱通过电化学调制触发，已经在球形红杆菌的细胞色素 c 氧化酶上进行了研究。使用相敏检测从酰胺 I 区域的宽带中分离出的单个带被归因于不同的氧化还原中心。它们在毫秒时间尺度上的吸光度变化可以拟合到以前建立的基于质子依赖的化学反应动力学的模型。揭示了与氧化还原跃迁相关的二级结构的大量构象变化。
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