通过内体运输调节Notch信号的遗传回路。

Genetic circuitry modulating notch signals through endosomal trafficking.

作者信息

Hori Kazuya, Sen Anindya, Artavanis-Tsakonas Spyros

机构信息

Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.

出版信息

Methods Enzymol. 2014;534:283-99. doi: 10.1016/B978-0-12-397926-1.00016-0.

DOI:10.1016/B978-0-12-397926-1.00016-0

PMID:24359960

Abstract

Genetic modifier screens offer a powerful, indeed a uniquely powerful tool for the analysis and identification of elements capable of modulating specific cellular functions in development. Here, we describe the methodology that allowed us to explore the genetic circuitry that affects a Notch mutant phenotype caused by the abnormal endosomal trafficking of the Notch receptor. Endosomal trafficking events are increasingly appreciated to play a major role in controlling Notch signaling in development.

摘要

遗传修饰筛选提供了一种强大的，实际上是独一无二的强大工具，用于分析和鉴定能够在发育过程中调节特定细胞功能的元件。在这里，我们描述了一种方法，该方法使我们能够探索影响由Notch受体内体运输异常导致的Notch突变表型的遗传通路。人们越来越认识到内体运输事件在发育过程中控制Notch信号传导方面发挥着重要作用。