Sun Yu-feng, Fan Yan-yan, Huang Yan-fei, Wang Mei, Lu Xin-xin
Department of Clinical Laboratory Medicine, Affiliated Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
Department of Clinical Laboratory Medicine, Affiliated Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China. Email:
Zhonghua Yi Xue Za Zhi. 2013 Sep 10;93(34):2733-5.
To explore drug resistance and virulence characteristics of clinically isolated Vibrio parahaemolyticus (VP).
The patient records were reviewed. The laboratory information system was employed to count the quantity of bacterial diarrhea pathogens at our intestinal clinic from 2009 to 2012. The drug resistant phenotype was tested by the method of disk diffusion. Polymerase chain reaction (PCR) was used to detect thermo-stable direct hemolysin (TDH) gene (tdh), TDH-related hemolysin gene (trh) and type III secretion system 2 (T3SS2α, T3SS2β).
The VP infection rate was 37.90% (274/723). Susceptibility test showed that the resistance rates of ampicillin, cefalotin, cefuroxime and amikacin were 100% (216/216), 44.91% (97/216), 24.07% (52/216), 9.26% (20/216). The other 11 kinds of antibiotics were sensitive or intermediate. 96.76% (209/216) VP harbored tdh gene, 1.39% (3/216) trh gene and 100% T3SS2 gene, with 213 strains T3SS2α positive, 2 strains T3SS2β positive and 1 strain both T3SS2α and T3SS2β positive.
The resistant rate of VP to ampicillin is the highest and VP has different levels of resistance against other antibiotics. Most clinical isolates of VP harbor tdh gene and T3SS2 plays an important role in the pathogenicity of VP.
探讨临床分离副溶血性弧菌（VP）的耐药性及毒力特征。
回顾患者病历。利用实验室信息系统统计2009年至2012年我院肠道门诊细菌性腹泻病原菌的数量。采用纸片扩散法检测耐药表型。应用聚合酶链反应（PCR）检测耐热直接溶血素（TDH）基因（tdh）、TDH相关溶血素基因（trh）及Ⅲ型分泌系统2（T3SS2α、T3SS2β）。
VP感染率为37.90%（274/723）。药敏试验显示，氨苄西林、头孢噻吩、头孢呋辛和阿米卡星的耐药率分别为100%（216/216）、44.91%（97/216）、24.07%（52/216）、9.26%（20/216）。其他11种抗生素敏感或中介。96.76%（209/216）的VP携带tdh基因，1.39%（3/216）携带trh基因，T3SS2基因携带率为100%，其中T3SS2α阳性213株，T3SS2β阳性2株，T3SS2α和T3SS2β均阳性1株。
VP对氨苄西林耐药率最高，对其他抗生素有不同程度耐药。多数临床分离株VP携带tdh基因，T3SS2在VP致病性中起重要作用。
