Department of Microbiology and Parasitology, University of Navarra, C/Irunlarrea 1, 31008 Pamplona, Spain.
Department of Microbiology and Parasitology, University of Navarra, C/Irunlarrea 1, 31008 Pamplona, Spain.
Int J Food Microbiol. 2014 Aug 1;184:109-12. doi: 10.1016/j.ijfoodmicro.2013.11.036. Epub 2013 Dec 7.
Controlling Listeria monocytogenes in smoked salmon is still a challenge for food business operators. The reported low levels and uneven distribution of the pathogen make it necessary to apply good sampling plans and analytical procedures in order to detect contaminated batches. However, analyses are expensive and due to current economic situations, usually only one sample per lot is checked instead of the n=5 samples established in Regulation (EC) No 2073/2005. This work proposes the investigation of L. monocytogenes by pooling pre-enriched Half Fraser from several units from a batch (n=6). The results obtained from a preliminary in-house validation (n=51 pools) showed that the performance characteristics (sensitivity and specificity) are very high (>95%). This high reliability, together with reduced costs (nearly half), suggests that the wet pooling test could be a good cost-effective approach for investigating L. monocytogenes in smoked salmon. Additional research is needed before this approach can be used for other food matrices, such as determining the maximum number of units per batch that can be pooled with effective detection of L. monocytogenes.
控制烟熏三文鱼中的单增李斯特菌仍然是食品企业经营者面临的挑战。据报道，该病原体的低水平和不均匀分布使得有必要应用良好的抽样计划和分析程序，以检测受污染的批次。然而，分析成本高昂，而且由于当前的经济形势，通常每个批次只检查一个样本，而不是按照 2005 年（EC）第 2073/2005 号法规规定的 n=5 个样本进行检查。本工作通过从一批次的多个单位中合并预富集 Half Fraser 来研究单增李斯特菌（n=6）。初步内部验证（n=51 个合并样本）的结果表明，性能特征（灵敏度和特异性）非常高（>95%）。这种高可靠性，加上成本降低（近一半），表明湿合并测试可能是一种用于调查烟熏三文鱼中单增李斯特菌的具有成本效益的良好方法。在这种方法可用于其他食品基质之前，如确定每个批次可以合并的最大单位数量，以有效检测单增李斯特菌，还需要进行更多的研究。
