Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Food Chem. 2014 May 1;150:260-6. doi: 10.1016/j.foodchem.2013.10.143. Epub 2013 Nov 2.
In this study, a rapid, simple and sensitive analytical method was developed for the quantitative determination of 20 nucleosides and nucleobases in functional foods at trace levels using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC-UPLC-TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Under optimised chromatographic conditions, good separation of 20 target compounds was achieved using a Waters Acquity UPLC BEH Amide column and gradient elution in 11min. The limits of detection (LODs) and quantification (LOQs) were between 0.02-42.54ng/mL and 0.05-98.18ng/mL for the 20 analytes, respectively. This is the first report about simultaneous analysis of nucleosides and nucleobases in functional foods using this method, which afforded good linearity, precision, repeatability and accuracy. The method developed was successfully applied to quantify target compounds in batches of ginkgo seeds. The method potentially could be used to determine polar and trace-level nucleosides and nucleobases in ginkgo seeds.
本研究建立了一种快速、简单、灵敏的亲水作用超高效液相色谱-串联三重四极杆质谱法（HILIC-UPLC-TQ-MS/MS）同时测定痕量水平下功能性食品中 20 种核苷和碱基的分析方法。在优化的色谱条件下，采用 Waters Acquity UPLC BEH Amide 柱，以 11min 梯度洗脱，可实现 20 种目标化合物的良好分离。20 种分析物的检出限（LOD）和定量限（LOQ）分别在 0.02-42.54ng/mL 和 0.05-98.18ng/mL 之间。这是首次采用该方法同时分析功能性食品中的核苷和碱基，该方法具有良好的线性、精密度、重复性和准确性。所建立的方法成功应用于银杏种子中目标化合物的定量分析。该方法有望用于测定银杏种子中的极性和痕量核苷和碱基。
