Nordström O, Melander T, Hökfelt T, Bartfai T, Goldstein M
Neurosci Lett. 1987 Jan 2;73(1):21-6. doi: 10.1016/0304-3940(87)90024-3.
The role of the neuropeptide galanin (GAL) in rat hypothalamus has been studied in different experimental models. Thus, the effect of GAL on potassium-induced dopamine release was analyzed in vitro, and the localization of GAL and GAL binding sites was studied with immunohistochemistry and receptor autoradiography, respectively. In the median eminence GAL and presumably dopamine were found to coexist in nerve endings and this area contained a high density of 125I-GAL binding sites. In vitro experiments revealed that GAL inhibited the release of [3H]dopamine in a dose-dependent manner (IC50 = 7-10 nM), possibly via a presynaptic receptor.
在不同的实验模型中，对神经肽甘丙肽（GAL）在大鼠下丘脑的作用进行了研究。因此，在体外分析了GAL对钾诱导的多巴胺释放的影响，并用免疫组织化学和受体放射自显影分别研究了GAL和GAL结合位点的定位。在正中隆起中，发现GAL与推测的多巴胺共存于神经末梢，并且该区域含有高密度的125I-GAL结合位点。体外实验表明，GAL以剂量依赖的方式抑制[3H]多巴胺的释放（IC50 = 7-10 nM），可能是通过突触前受体实现的。
