Bandler R, Tork I
Neurosci Lett. 1987 Feb 10;74(1):1-6. doi: 10.1016/0304-3940(87)90041-3.
Wheat germ agglutinin-horseradish peroxidase (WGA-HRP) injections were made at the same sites within the midbrain periaqueductal grey region (PAG) of the cat at which microinjections of excitatory amino acids had previously elicited the set of autonomic and somatic reactions (i.e. pupil dilatation, piloerection, retraction of the ears, arching of the back, hissing, howling and growling) known as the 'defence reaction'. The WGA-HRP injections revealed that this PAG region has an extensive set of afferent and efferent connections with solitary tract nuclei (NTS). Within the NTS the majority of labelled neurons were distributed, in approximately equal numbers, in the ipsilateral medial solitary nucleus (SM) and the ipsilateral and the contralateral ventrolateral solitary nuclei (SVL). The densest anterograde labelling was found in the ipsilateral SM, with lighter anterograde labelling in the contralateral SM and bilaterally in the commissural solitary nucleus and SVL. The described connections between the defence region of the PAG and the NTS not only provide a new anatomical basis for cardiovascular and respiratory components of the reaction, but also add to the evidence that, in addition to the hypothalamus, the PAG is an important integrating center for the autonomic and somatic elements of the defence reaction.
将小麦胚凝集素-辣根过氧化物酶（WGA-HRP）注射到猫中脑导水管周围灰质区域（PAG）内与先前微量注射兴奋性氨基酸引发一组自主和躯体反应（即瞳孔扩张、竖毛、耳朵后缩、背部拱起、嘶嘶声、嚎叫和咆哮）（即所谓的“防御反应”）相同的部位。WGA-HRP注射显示，该PAG区域与孤束核（NTS）有广泛的传入和传出连接。在NTS内，大多数标记神经元以大致相等的数量分布在同侧内侧孤束核（SM）以及同侧和对侧腹外侧孤束核（SVL）中。在同侧SM中发现最密集的顺行标记，在对侧SM中顺行标记较轻，在连合孤束核和SVL中双侧顺行标记较轻。PAG防御区域与NTS之间所述的连接不仅为该反应的心血管和呼吸成分提供了新的解剖学基础，而且还进一步证明，除下丘脑外，PAG是防御反应自主和躯体成分的重要整合中心。
