AmorePacific Corporation R&D Unit, Yongin-si, Republic of Korea.
AmorePacific Corporation R&D Unit, Yongin-si, Republic of Korea; Division of Chemical Engineering, College of Engineering, Hanyang University, Seoul, Republic of Korea.
Toxicol Lett. 2014 Feb 10;225(1):185-91. doi: 10.1016/j.toxlet.2013.12.007. Epub 2013 Dec 19.
Cosmetics are normally composed of various ingredients. Some cosmetic ingredients can act as chemical haptens reacting toward proteins or peptides of human skin and they can provoke an immunologic reaction, called as skin sensitization. This haptenation process is very important step of inducing skin sensitization and evaluating the sensitizing potentials of cosmetic ingredients is very important for consumer safety. Therefore, animal alternative methods focusing on monitoring haptenation potential are undergoing vigorous research. To examine the further usefulness of spectrophotometric methods to monitor reactivity of chemicals toward peptides for cosmetic ingredients. Forty chemicals (25 sensitizers and 15 non-sensitizers) were reacted with 2 synthetic peptides, e.g., the cysteine peptides (Ac-RFAACAA-COOH) with free thiol group and the lysine peptides (Ac-RFAAKAA-COOH) with free amine group. Unreacted peptides can be detected after incubating with 5,5'-dithiobis-2-nitrobenzoic acid or fluorescamine™ as detection reagents for free thiol and amine group, respectively. Chemicals were categorized as sensitizers when they induced more than 10% depletion of cysteine peptides or more than 30% depletion of lysine peptides. The sensitivity, specificity, and accuracy were 80.0%, 86.7% and 82.5%, respectively. These results demonstrate that spectrophotometric methods can be an easy, fast, and high-throughput screening tools predicting the skin sensitization potential of chemical including cosmetic ingredient.
化妆品通常由各种成分组成。一些化妆品成分可以作为化学半抗原，与人体皮肤的蛋白质或肽发生反应，从而引发免疫反应，称为皮肤致敏。这种半抗原化过程是诱导皮肤致敏的重要步骤，评估化妆品成分的致敏潜力对于消费者安全非常重要。因此，专注于监测半抗原化潜力的动物替代方法正在进行激烈的研究。为了研究分光光度法监测化妆品成分中化学物质与肽反应性的进一步用途。将 40 种化学物质（25 种致敏剂和 15 种非致敏剂）与 2 种合成肽（具有游离巯基的半胱氨酸肽（Ac-RFAACAA-COOH）和具有游离氨基的赖氨酸肽（Ac-RFAAKAA-COOH））反应。用 5,5'-二硫代双-2-硝基苯甲酸或荧光胺™作为检测游离巯基和氨基的检测试剂，可检测未反应的肽。当化学物质导致半胱氨酸肽的消耗超过 10%或赖氨酸肽的消耗超过 30%时，将其归类为致敏剂。灵敏度、特异性和准确性分别为 80.0%、86.7%和 82.5%。这些结果表明，分光光度法可以作为一种简单、快速、高通量的筛选工具，预测包括化妆品成分在内的化学物质的皮肤致敏潜力。
