Division of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China; Nano Biomedical Research Center, School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, PR China.
Nano Biomedical Research Center, School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, PR China.
Clin Biochem. 2014 Feb;47(3):220-6. doi: 10.1016/j.clinbiochem.2013.12.010. Epub 2013 Dec 18.
OBJECTIVES: Magnetic nanoparticles (MNPs) are important carriers in immunoassays. In this study, we investigated the influence of the physical and chemical properties of MNPs on their performance in a detection process. DESIGN AND METHODS: A comparative study of the properties of two MNPs with sizes of 200nm and 1μm (MNP-200nm and MNP-1μm, respectively) was conducted using the following four aspects: nonspecific adsorption to proteins (IgG was used as a model protein), influence of magnetic nanoparticles on the chemiluminescence signal, response speed to an external magnetic field, and intensity of the detection signal. RESULTS: MNP-1μm exhibited lower nonspecific adsorption to IgG in serum, a weaker interference with the chemiluminescence signal, and a higher response speed to the external magnetic field than the same amount of MNP-200nm. An automated chemiluminescence immunoassay system based on MNP-1μm was also established. CONCLUSIONS: MNP-1μm acts as an excellent carrier in an automated chemiluminescence immunoassay system for the analysis of serum samples from clinical patients.
目的：磁性纳米颗粒（MNPs）是免疫测定中的重要载体。本研究旨在探讨 MNPs 的物理化学性质对其在检测过程中的性能的影响。
设计与方法：通过以下四个方面对两种粒径为 200nm 和 1μm 的 MNPs（分别为 MNP-200nm 和 MNP-1μm）的性质进行了比较研究：对蛋白质的非特异性吸附（IgG 用作模型蛋白）、对化学发光信号的影响、对外磁场的响应速度以及检测信号的强度。
结果：与相同数量的 MNP-200nm 相比，MNP-1μm 在血清中对 IgG 的非特异性吸附较低，对化学发光信号的干扰较弱，对外磁场的响应速度较快。还建立了基于 MNP-1μm 的自动化化学发光免疫分析系统。
结论：MNP-1μm 可作为临床患者血清样本分析的自动化化学发光免疫分析系统中的优良载体。
Bioengineering (Basel). 2019-8-28
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