Department of Orthopaedics, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Anal Chim Acta. 2012 Apr 13;722:114-8. doi: 10.1016/j.aca.2012.02.007. Epub 2012 Feb 13.
To detect a biomarker for small cell lung carcinoma, neuron specific enolase (NSE), a sensitive and specific chemiluminescence enzyme immunoassay was developed. Fluorescein isothiocyanate (FITC) labeled NSE capture antibody connected with NSE and alkaline phosphatase (ALP) labeled NSE detection antibody in a sandwich-type detection manner. This immune complex was further reacted with anti-FITC coated magnetic beads. In a magnetic field, the complex was enriched, and the sensitivity was thus enhanced. The limit of detection (LOD) of this method was <0.2 ng mL(-1). The proposed immunoassay was highly selective, and not interfered by hook effect. The recovery was >83.0% and the coefficient of variation was <10.0%. Human sera from 120 patients were tested with the presented and traditional chemiluminescence enzyme immunoassay. An excellent linear relationship was obtained between two techniques. Overall, this immunoassay offers a promising alternative for NSE detection than traditional clinical examinations.
为了检测小细胞肺癌的生物标志物，神经元特异性烯醇化酶（NSE），开发了一种灵敏且特异的化学发光酶免疫分析方法。荧光素异硫氰酸酯（FITC）标记的 NSE 捕获抗体与 NSE 结合，碱性磷酸酶（ALP）标记的 NSE 检测抗体以三明治式检测方式结合。该免疫复合物进一步与抗 FITC 包被的磁珠反应。在磁场中，复合物被富集，从而提高了灵敏度。该方法的检测限（LOD）<0.2ng mL(-1)。该免疫分析方法具有高度选择性，不受钩状效应干扰。回收率>83.0%，变异系数<10.0%。用本方法和传统化学发光酶免疫分析法检测了 120 例患者的血清。两种技术之间获得了极好的线性关系。总的来说，该免疫分析方法为 NSE 检测提供了一种有前途的替代传统临床检查的方法。
