Schrenk P, Kobinia G S, Brücke P, Syre G, Edtstadler A
Thorac Cardiovasc Surg. 1987 Feb;35(1):6-10. doi: 10.1055/s-2007-1020188.
A laboratory study was undertaken to improve the initial count of endothelial cells (EC) adhering to the wall of e-PTFE prostheses when seeding of human EC is attempted. In our experiments pretreatment of the prosthetic wall with commercially available fibrin glue (Tissucol) improved the reliability of the seeding procedure. The number and the distribution of EC seeded onto fibrin glue presealed e-PTFE prostheses was compared to the number and distribution of EC adhering to blood preclotted grafts 24 hours following the initial seeding procedure. Fibrin glue presealed grafts showed a higher number of initially adhering EC and a more equal distribution over the graft surface when compared to blood pretreated grafts. Our results suggest that the use of fibrin glue enhances the seeding of human EC on e-PTFE grafts.
进行了一项实验室研究，旨在尝试接种人内皮细胞（EC）时提高附着于e-PTFE人工血管壁上的内皮细胞的初始计数。在我们的实验中，用市售纤维蛋白胶（Tissucol）对人工血管壁进行预处理提高了接种程序的可靠性。将接种到纤维蛋白胶预密封的e-PTFE人工血管上的EC数量和分布与初始接种程序后24小时附着于血液预凝移植物上的EC数量和分布进行比较。与血液预处理的移植物相比，纤维蛋白胶预密封的移植物显示出更多初始附着的EC，并且在移植物表面分布更均匀。我们的结果表明，使用纤维蛋白胶可增强人EC在e-PTFE移植物上的接种。
