Mosevich T N, Komissarchik Ia Iu, Ugolev A M
Tsitologiia. 1987 Jan;29(1):22-7.
A study was made of the structural changes in different organoids of enterocytes of the rat small intestine at "rest" and during lipid absorption using ultrathin sections, impregnation with Ur-Pb-Cu, and continuous impregnation with OsO4. With the latter technique a specific canalicular system was found in the enterocytes, which we failed to observe on ultrathin sections. During lipid absorption the canalicular system underwent fragmentation. The vesicules on the trans-surface of the Golgi apparatus were enlarged, and the number of flattened cisterns was significantly lower. The changes in cellular organoids and their interrelations observed in the present study support the earlier hypothesis of the resynthesis of triglycerides within the enterocytes, the formation of chylomicrons and their transfer into the intercellular space.
利用超薄切片、铀-铅-铜浸染以及四氧化锇连续浸染技术，对大鼠小肠肠上皮细胞处于“静止”状态及脂质吸收过程中不同细胞器的结构变化进行了研究。采用后一种技术，在肠上皮细胞中发现了一种特殊的小管系统，而在超薄切片上未观察到。在脂质吸收过程中，小管系统发生碎片化。高尔基体反面膜囊上的小泡增大，扁平潴泡的数量显著减少。本研究中观察到的细胞器变化及其相互关系支持了早期关于肠上皮细胞内甘油三酯再合成、乳糜微粒形成及其向细胞间隙转移的假说。
