利用重复序列聚合酶链反应对苏云金芽孢杆菌进行分子鉴定
Molecular characterization of Bacillus thuringiensis using rep-PCR.
作者信息
da Silva Rosane Bezerra, Valicente Fernando Hercos
机构信息
Department of Plant Biotechnology, Federal University of Lavras, Lavras, Minas Gerais Brazil.
Department of Biological control, Embrapa Maize and Sorghum research, Sete Lagoas, Minas Gerais Brazil.
出版信息
Springerplus. 2013 Nov 29;2:641. doi: 10.1186/2193-1801-2-641. eCollection 2013.
The genetic divergence of 65 strains of Bacillus thuringiensis (Bt) was determined using Rep-PCR. Based on the repetitive sequences the BOX primer was the most informative with 26 fragments, followed by ERIC (19) and REP (10), generating a total of 55 fragments. The dendogram shows that ten groups were formed when 45% was the average distance of the population: group 1 with 41,5% of the isolates, 33,8% of the isolates were distributed in other groups and 24,6% did not formed distinct group. 53,2% of the isolates from Embrapa are in the group 1, and 29,8% of the isolates are distributed in other groups. Bt strains from USDA and Institute Pasteur showed more variability.
使用重复序列聚合酶链反应（Rep-PCR）测定了65株苏云金芽孢杆菌（Bt）的遗传差异。基于重复序列，BOX引物信息量最大，产生了26个片段，其次是ERIC引物（19个片段）和REP引物（10个片段），总共产生了55个片段。聚类图显示，当群体平均距离为45%时形成了10个组：第1组包含41.5%的分离株，33.8%的分离株分布在其他组，24.6%未形成明显的组。巴西农牧业研究公司（Embrapa）的分离株中有53.2%在第1组，29.8%的分离株分布在其他组。美国农业部（USDA）和巴斯德研究所的Bt菌株表现出更大的变异性。
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