Stern M J, Ames G F, Smith N H, Robinson E C, Higgins C F
Cell. 1984 Jul;37(3):1015-26. doi: 10.1016/0092-8674(84)90436-7.
We describe a remarkably conserved nucleotide sequence, the many copies of which may occupy up to 1% of the genomes of E. coli and S. typhimurium. This sequence, the REP (repetitive extragenic palindromic) sequence, is about 35 nucleotides long, includes an inverted repeat, and can occur singly or in multiple adjacent copies. A possible role for the REP sequences in regulation of gene expression has been thoroughly investigated. While the REP sequences do not appear to modulate differential gene expression within an operon, they can affect the expression of both upstream and downstream genes to a small extent, probably by affecting the rate of mRNA degradation. Possible roles for the REP sequence in mRNA degradation, chromosome structure, and recombination are discussed.
我们描述了一种高度保守的核苷酸序列，其多个拷贝可能占据大肠杆菌和鼠伤寒沙门氏菌基因组的1%。这个序列，即REP（重复基因外回文序列）序列，大约35个核苷酸长，包含一个反向重复序列，可以单个出现或多个相邻拷贝出现。REP序列在基因表达调控中的可能作用已得到深入研究。虽然REP序列似乎不会调节操纵子内的差异基因表达，但它们可以在一定程度上影响上游和下游基因的表达，可能是通过影响mRNA降解速率来实现的。文中讨论了REP序列在mRNA降解、染色体结构和重组中的可能作用。
