Ciarkowska Anna, Jakubowska Anna
Department of Biochemistry, Nicolaus Copernicus University, 1 Lwowska St., 87-100 Toruń, Poland.
Postepy Biochem. 2013;59(3):315-21.
Pichia pastoris has become increasingly popular as a host for recombinant protein production in recent years. P. pastoris is more cost effective and allows achieving higher expression levels than insect and mammalian cells. It also offers some significant advantages over E. coli expression systems, such as avoiding problems with proper protein folding. Also, P. pastoris as an eukaryotic organism can carry out posttranslational modifications of produced proteins. Additionally, P. pastoris can produce high levels of recombinant proteins in extracellular medium which simplifies protein purification. Having many advantages over other expression systems makes P. pastoris an organism of choice for industrial protein production.
近年来，毕赤酵母作为重组蛋白生产的宿主越来越受欢迎。与昆虫细胞和哺乳动物细胞相比，毕赤酵母更具成本效益，能够实现更高的表达水平。与大肠杆菌表达系统相比，它还具有一些显著优势，比如避免了蛋白质正确折叠方面的问题。此外，毕赤酵母作为一种真核生物，可以对所产生的蛋白质进行翻译后修饰。另外，毕赤酵母能够在细胞外培养基中高水平表达重组蛋白，这简化了蛋白质的纯化过程。与其他表达系统相比具有诸多优势，使得毕赤酵母成为工业蛋白生产的首选生物。
