Department of Applied Chemistry, China Agricultural University , Yuanmingyuan West Road 2, Beijing 100193, China.
J Agric Food Chem. 2014 Jan 15;62(2):360-7. doi: 10.1021/jf403996g. Epub 2013 Dec 31.
In this study, the enantioselective bioaccumulation of epoxiconazole enantiomers in tubifex (Oligochaeta, Tubificida) was investigated in two uptake pathways. A sensitive and rapid chiral method was developed for the determination of epoxiconazole enantiomers in tubifex and soil based on high-performance liquid chromatography coupled with triple-quadrupole mass spectrometry (HPLC-MS/MS). In the spiked-water or spiked-soil treatments, enantioselective bioaccumulation of epoxiconazole in tubifex was obersved. For spiked-water treatment, (-)-epoxiconazole accumulated in tubifex to a greater extent than (+)-epoxiconazole, leading to enrichments with a composition (-) > (+). However, for spiked-soil treatment, the enantioselectivity in tubifex was reversed with a preferential accumulation of (+)-epoxiconazole. Calculated accumulation factors (AFs) indicated that epoxiconazole in spiked-water treatment had higher bioaccumulation potential than that in spiked-soil treatment. The results from the spiked-soil treatment also revealed that the dissipation of epoxiconazole in soil was enantioselective, and tubifex has positive effects on epoxiconazole diffusion from soil to overlying water.
在这项研究中，通过两种吸收途径研究了映喃唑对映体在水丝蚓（寡毛纲，颤蚓目）中的对映体选择性生物累积。建立了一种基于高效液相色谱-串联三重四极杆质谱（HPLC-MS/MS）测定水丝蚓和土壤中映喃唑对映体的灵敏、快速的手性方法。在添加水或添加土壤的处理中，观察到映喃唑在水丝蚓中的对映体选择性生物累积。对于添加水的处理，（-）-映喃唑在水丝蚓中的积累程度大于（+）-映喃唑，导致组成（-）>（+）富集。然而，对于添加土壤的处理，水丝蚓中的对映体选择性发生反转，（+）-映喃唑优先积累。计算的积累因子（AF）表明，添加水中的映喃唑比添加土壤中的映喃唑具有更高的生物累积潜力。添加土壤处理的结果还表明，土壤中映喃唑的消解具有对映体选择性，水丝蚓对映喃唑从土壤向覆盖水的扩散具有积极影响。
